Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
234 | 5' | -50.9 | AC_000008.1 | + | 26881 | 0.66 | 0.807975 |
Target: 5'- -gGGGGcGUGGUCUACAGccGCGUCugGAa -3' miRNA: 3'- cgUCUC-CACUGGAUGUU--CGUAGugCU- -5' |
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234 | 5' | -50.9 | AC_000008.1 | + | 15047 | 0.67 | 0.744023 |
Target: 5'- gGCGGGGGUGGC--GCAGGCGgcaGCa- -3' miRNA: 3'- -CGUCUCCACUGgaUGUUCGUag-UGcu -5' |
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234 | 5' | -50.9 | AC_000008.1 | + | 6489 | 0.69 | 0.652061 |
Target: 5'- aGCAGAGGcGGCCgcccuugcGCGAGCAgaaUgGCGGu -3' miRNA: 3'- -CGUCUCCaCUGGa-------UGUUCGU---AgUGCU- -5' |
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234 | 5' | -50.9 | AC_000008.1 | + | 1144 | 0.7 | 0.581808 |
Target: 5'- uGCGaAGG-GACCUACGGG-AUCGCGGu -3' miRNA: 3'- -CGUcUCCaCUGGAUGUUCgUAGUGCU- -5' |
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234 | 5' | -50.9 | AC_000008.1 | + | 8549 | 0.71 | 0.547173 |
Target: 5'- cGCGGGGGUGuCCUugGAugauGCAUCuaaaaGCGGu -3' miRNA: 3'- -CGUCUCCACuGGAugUU----CGUAG-----UGCU- -5' |
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234 | 5' | -50.9 | AC_000008.1 | + | 12659 | 1.12 | 0.001007 |
Target: 5'- uGCAGAGGUGACCUACAAGCAUCACGAg -3' miRNA: 3'- -CGUCUCCACUGGAUGUUCGUAGUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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