miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
234 5' -50.9 AC_000008.1 + 26881 0.66 0.807975
Target:  5'- -gGGGGcGUGGUCUACAGccGCGUCugGAa -3'
miRNA:   3'- cgUCUC-CACUGGAUGUU--CGUAGugCU- -5'
234 5' -50.9 AC_000008.1 + 15047 0.67 0.744023
Target:  5'- gGCGGGGGUGGC--GCAGGCGgcaGCa- -3'
miRNA:   3'- -CGUCUCCACUGgaUGUUCGUag-UGcu -5'
234 5' -50.9 AC_000008.1 + 6489 0.69 0.652061
Target:  5'- aGCAGAGGcGGCCgcccuugcGCGAGCAgaaUgGCGGu -3'
miRNA:   3'- -CGUCUCCaCUGGa-------UGUUCGU---AgUGCU- -5'
234 5' -50.9 AC_000008.1 + 1144 0.7 0.581808
Target:  5'- uGCGaAGG-GACCUACGGG-AUCGCGGu -3'
miRNA:   3'- -CGUcUCCaCUGGAUGUUCgUAGUGCU- -5'
234 5' -50.9 AC_000008.1 + 8549 0.71 0.547173
Target:  5'- cGCGGGGGUGuCCUugGAugauGCAUCuaaaaGCGGu -3'
miRNA:   3'- -CGUCUCCACuGGAugUU----CGUAG-----UGCU- -5'
234 5' -50.9 AC_000008.1 + 12659 1.12 0.001007
Target:  5'- uGCAGAGGUGACCUACAAGCAUCACGAg -3'
miRNA:   3'- -CGUCUCCACUGGAUGUUCGUAGUGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.