Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2340 | 3' | -50.5 | NC_001416.1 | + | 38552 | 0.66 | 0.901458 |
Target: 5'- uCUCACCAaUAaaaaacgcCCGGcgGCAACCGa-- -3' miRNA: 3'- -GAGUGGUaAU--------GGCCuaUGUUGGCcuu -5' |
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2340 | 3' | -50.5 | NC_001416.1 | + | 20476 | 0.66 | 0.894128 |
Target: 5'- -cCGCCGUUGCCGaGGaagcugcACGACCGGc- -3' miRNA: 3'- gaGUGGUAAUGGC-CUa------UGUUGGCCuu -5' |
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2340 | 3' | -50.5 | NC_001416.1 | + | 4739 | 0.66 | 0.894128 |
Target: 5'- gCUgACCAgcgUGCCGGAcaccgGCAGCaCGGc- -3' miRNA: 3'- -GAgUGGUa--AUGGCCUa----UGUUG-GCCuu -5' |
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2340 | 3' | -50.5 | NC_001416.1 | + | 8567 | 0.67 | 0.844226 |
Target: 5'- --aGCCG-UACUGGAUGC-ACUGGAGa -3' miRNA: 3'- gagUGGUaAUGGCCUAUGuUGGCCUU- -5' |
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2340 | 3' | -50.5 | NC_001416.1 | + | 15753 | 0.69 | 0.764425 |
Target: 5'- gCUCACCAgc-CCGGA-ACAccaccgugacACCGGAu -3' miRNA: 3'- -GAGUGGUaauGGCCUaUGU----------UGGCCUu -5' |
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2340 | 3' | -50.5 | NC_001416.1 | + | 40794 | 0.69 | 0.764425 |
Target: 5'- gUCGCUg--GCUGGAUGCAgaaaGCUGGAAg -3' miRNA: 3'- gAGUGGuaaUGGCCUAUGU----UGGCCUU- -5' |
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2340 | 3' | -50.5 | NC_001416.1 | + | 4657 | 0.69 | 0.739417 |
Target: 5'- aUCGCCGUgcUGCCGGuguccggcacgcugGU-CAGCCGGAc -3' miRNA: 3'- gAGUGGUA--AUGGCC--------------UAuGUUGGCCUu -5' |
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2340 | 3' | -50.5 | NC_001416.1 | + | 3359 | 0.7 | 0.716027 |
Target: 5'- -gCAUCAgcaacccgaacaaUACCGGcgACAGCCGGAAc -3' miRNA: 3'- gaGUGGUa------------AUGGCCuaUGUUGGCCUU- -5' |
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2340 | 3' | -50.5 | NC_001416.1 | + | 8863 | 0.7 | 0.675059 |
Target: 5'- aUCGCuCAUcACCGGAUAaauCCGGGAc -3' miRNA: 3'- gAGUG-GUAaUGGCCUAUguuGGCCUU- -5' |
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2340 | 3' | -50.5 | NC_001416.1 | + | 21744 | 0.71 | 0.66355 |
Target: 5'- gUCACuCAUUGuCCGG-UACAGCCGuGAGu -3' miRNA: 3'- gAGUG-GUAAU-GGCCuAUGUUGGC-CUU- -5' |
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2340 | 3' | -50.5 | NC_001416.1 | + | 22217 | 0.71 | 0.613843 |
Target: 5'- aCUCGguCCGUUACCGGAaaauuuuaccugguUAUcGCCGGGAg -3' miRNA: 3'- -GAGU--GGUAAUGGCCU--------------AUGuUGGCCUU- -5' |
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2340 | 3' | -50.5 | NC_001416.1 | + | 15086 | 0.79 | 0.238892 |
Target: 5'- -cCGCCAUUGCCGGAucauucuuUACcGCCGGAGc -3' miRNA: 3'- gaGUGGUAAUGGCCU--------AUGuUGGCCUU- -5' |
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2340 | 3' | -50.5 | NC_001416.1 | + | 9695 | 1.07 | 0.003335 |
Target: 5'- aCUCACCAUUACCGGAUACAACCGGAAu -3' miRNA: 3'- -GAGUGGUAAUGGCCUAUGUUGGCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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