Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23402 | 3' | -54.1 | NC_005259.1 | + | 28967 | 0.7 | 0.625769 |
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Target: 5'- cCGAGgUCGgUGCCGAGgcCG-CGAGc -3' miRNA: 3'- uGCUCgAGCaACGGCUCauGCuGCUC- -5' |
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| 23402 | 3' | -54.1 | NC_005259.1 | + | 21216 | 0.7 | 0.625769 |
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Target: 5'- cCGAGCUCauccgaGCCGAGUACGccucggcagcgcGCGGGu -3' miRNA: 3'- uGCUCGAGcaa---CGGCUCAUGC------------UGCUC- -5' |
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| 23402 | 3' | -54.1 | NC_005259.1 | + | 67218 | 0.7 | 0.614922 |
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Target: 5'- gGCGGGgUCGaUGCCGAccACGGCGGu -3' miRNA: 3'- -UGCUCgAGCaACGGCUcaUGCUGCUc -5' |
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| 23402 | 3' | -54.1 | NC_005259.1 | + | 28755 | 0.7 | 0.593281 |
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Target: 5'- cCGAGCgugagaUCGUcGCCG-GUGcCGACGAGg -3' miRNA: 3'- uGCUCG------AGCAaCGGCuCAU-GCUGCUC- -5' |
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| 23402 | 3' | -54.1 | NC_005259.1 | + | 5063 | 0.72 | 0.508604 |
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Target: 5'- uCGAGaUCGUggGCCGGGU-CGACGGGc -3' miRNA: 3'- uGCUCgAGCAa-CGGCUCAuGCUGCUC- -5' |
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| 23402 | 3' | -54.1 | NC_005259.1 | + | 8431 | 0.73 | 0.478124 |
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Target: 5'- uGCGAuGCUCGUUGCCGguggucucGGUGUGGuCGAGg -3' miRNA: 3'- -UGCU-CGAGCAACGGC--------UCAUGCU-GCUC- -5' |
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| 23402 | 3' | -54.1 | NC_005259.1 | + | 19201 | 0.73 | 0.42944 |
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Target: 5'- aGCGAGCUCGUggUGCgGGcgGCGACGuGg -3' miRNA: 3'- -UGCUCGAGCA--ACGgCUcaUGCUGCuC- -5' |
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| 23402 | 3' | -54.1 | NC_005259.1 | + | 7317 | 0.74 | 0.410798 |
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Target: 5'- cCGAGUUcaccgcCGUcGCCGAGUACGuCGAGg -3' miRNA: 3'- uGCUCGA------GCAaCGGCUCAUGCuGCUC- -5' |
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| 23402 | 3' | -54.1 | NC_005259.1 | + | 65695 | 0.79 | 0.212203 |
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Target: 5'- gACGAGCgg---GCCGAGUAgGACGAGg -3' miRNA: 3'- -UGCUCGagcaaCGGCUCAUgCUGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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