Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23403 | 5' | -59.2 | NC_005259.1 | + | 8858 | 0.66 | 0.52895 |
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Target: 5'- -aCAGCgugCcCGCCGCCUGCCGc---- -3' miRNA: 3'- ucGUCGa--GaGCGGCGGGCGGCuuaua -5' |
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| 23403 | 5' | -59.2 | NC_005259.1 | + | 44231 | 0.66 | 0.518645 |
|
Target: 5'- --gAGCUCgcccgccgCGCCGCCCGCgcgCGAGa-- -3' miRNA: 3'- ucgUCGAGa-------GCGGCGGGCG---GCUUaua -5' |
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| 23403 | 5' | -59.2 | NC_005259.1 | + | 37411 | 0.66 | 0.518645 |
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Target: 5'- uGUuGUUgaCGCCGCCCGCCGu---- -3' miRNA: 3'- uCGuCGAgaGCGGCGGGCGGCuuaua -5' |
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| 23403 | 5' | -59.2 | NC_005259.1 | + | 43239 | 0.66 | 0.518645 |
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Target: 5'- gAGCgAGCUgacgaUGCCGCCCgcGCCGggUGc -3' miRNA: 3'- -UCG-UCGAga---GCGGCGGG--CGGCuuAUa -5' |
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| 23403 | 5' | -59.2 | NC_005259.1 | + | 2161 | 0.66 | 0.508421 |
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Target: 5'- cAGCGGCgUCgaGCCGCCgguUGCCGAGg-- -3' miRNA: 3'- -UCGUCG-AGagCGGCGG---GCGGCUUaua -5' |
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| 23403 | 5' | -59.2 | NC_005259.1 | + | 30148 | 0.66 | 0.508421 |
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Target: 5'- gGGCAGCUCgaccggGCCGCCUacagGCCGc---- -3' miRNA: 3'- -UCGUCGAGag----CGGCGGG----CGGCuuaua -5' |
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| 23403 | 5' | -59.2 | NC_005259.1 | + | 60066 | 0.66 | 0.498282 |
|
Target: 5'- gGGCucaGGCUCggGCgGCUCGCCGAc--- -3' miRNA: 3'- -UCG---UCGAGagCGgCGGGCGGCUuaua -5' |
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| 23403 | 5' | -59.2 | NC_005259.1 | + | 9082 | 0.66 | 0.498282 |
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Target: 5'- cAGCauaGGC-C-CGuuGCCCGCCGAGg-- -3' miRNA: 3'- -UCG---UCGaGaGCggCGGGCGGCUUaua -5' |
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| 23403 | 5' | -59.2 | NC_005259.1 | + | 66276 | 0.66 | 0.549777 |
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Target: 5'- cGGCGGCUCaCGCCGCCaccauccacucUGCgCGcAUGUg -3' miRNA: 3'- -UCGUCGAGaGCGGCGG-----------GCG-GCuUAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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