Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23404 | 3' | -54.6 | NC_005259.1 | + | 4900 | 0.66 | 0.80432 |
Target: 5'- gGGUGAUcg-GAUGAGCUGCuuugCCGuAGGg -3' miRNA: 3'- -CUACUGcuaCUACUCGACG----GGC-UCCa -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 11123 | 0.66 | 0.794699 |
Target: 5'- cGGUGACGGUGc-GAGCcgucGCCUGcGGGUa -3' miRNA: 3'- -CUACUGCUACuaCUCGa---CGGGC-UCCA- -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 31668 | 0.66 | 0.774961 |
Target: 5'- -uUGACcucGAcgGGGUUGCCCGAGGc -3' miRNA: 3'- cuACUGcuaCUa-CUCGACGGGCUCCa -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 23043 | 0.66 | 0.764866 |
Target: 5'- --cGACGAc---GAGCUGCCCggcGAGGg -3' miRNA: 3'- cuaCUGCUacuaCUCGACGGG---CUCCa -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 43361 | 0.67 | 0.733812 |
Target: 5'- cGAUGAUGuacuUGAUGAGC-GCCUGAa-- -3' miRNA: 3'- -CUACUGCu---ACUACUCGaCGGGCUcca -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 64394 | 0.67 | 0.733812 |
Target: 5'- uGUGGguCGGUGAUGAGCUgcgaugcaGCCgUGAGGg -3' miRNA: 3'- cUACU--GCUACUACUCGA--------CGG-GCUCCa -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 25973 | 0.67 | 0.733812 |
Target: 5'- ---uGCGAUGAgaccGGGCaGCgCCGAGGUg -3' miRNA: 3'- cuacUGCUACUa---CUCGaCG-GGCUCCA- -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 19241 | 0.67 | 0.727481 |
Target: 5'- --cGGCGAUGAUcgauggccgccugccGGGCcGCCCGAuGGa -3' miRNA: 3'- cuaCUGCUACUA---------------CUCGaCGGGCU-CCa -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 37501 | 0.68 | 0.691036 |
Target: 5'- --cGGCGGUGAgcacGAuGCUGCCCGAc-- -3' miRNA: 3'- cuaCUGCUACUa---CU-CGACGGGCUcca -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 36931 | 0.68 | 0.669263 |
Target: 5'- --cGACGGUGAgUGuuGCUGUcaCCGGGGUg -3' miRNA: 3'- cuaCUGCUACU-ACu-CGACG--GGCUCCA- -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 12687 | 0.7 | 0.57073 |
Target: 5'- gGAUGACGAggGA-GAGUgGCUCGGGGa -3' miRNA: 3'- -CUACUGCUa-CUaCUCGaCGGGCUCCa -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 39128 | 0.7 | 0.57073 |
Target: 5'- --cGACaucGGUGAGCUGCCCGAuGUg -3' miRNA: 3'- cuaCUGcuaCUACUCGACGGGCUcCA- -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 28136 | 0.71 | 0.517306 |
Target: 5'- --cGACGuaggcGAUGAGCUGCCCGGc-- -3' miRNA: 3'- cuaCUGCua---CUACUCGACGGGCUcca -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 46434 | 0.71 | 0.476052 |
Target: 5'- cGUGACGAUGGUGgcaAGCUGCUgcucggUGAGGc -3' miRNA: 3'- cUACUGCUACUAC---UCGACGG------GCUCCa -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 14646 | 0.73 | 0.372107 |
Target: 5'- cGUGACGAUGGcgUGGGCUcaGCCCGAcucGGg -3' miRNA: 3'- cUACUGCUACU--ACUCGA--CGGGCU---CCa -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 8780 | 0.75 | 0.29932 |
Target: 5'- --cGGCGGUGAUGAGCUcgGCCCGcuuGGc -3' miRNA: 3'- cuaCUGCUACUACUCGA--CGGGCu--CCa -5' |
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23404 | 3' | -54.6 | NC_005259.1 | + | 37624 | 0.78 | 0.182646 |
Target: 5'- uGAUGGgGAUGAUGuGCUGCCCGAu-- -3' miRNA: 3'- -CUACUgCUACUACuCGACGGGCUcca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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