Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23406 | 3' | -55.8 | NC_005259.1 | + | 44247 | 0.69 | 0.54098 |
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Target: 5'- cCGCCCGCGCgCGAgAAGUcggCGAcaUCGa -3' miRNA: 3'- -GCGGGCGCGaGCUgUUCGa--GUU--AGCa -5' |
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| 23406 | 3' | -55.8 | NC_005259.1 | + | 496 | 0.7 | 0.530516 |
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Target: 5'- aCGCCaCGCuGCUCGACGGcauguucgauGCgCAGUCGg -3' miRNA: 3'- -GCGG-GCG-CGAGCUGUU----------CGaGUUAGCa -5' |
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| 23406 | 3' | -55.8 | NC_005259.1 | + | 36880 | 0.7 | 0.530516 |
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Target: 5'- cCGCCCGCGC-CGACGAcGC-CGGUg-- -3' miRNA: 3'- -GCGGGCGCGaGCUGUU-CGaGUUAgca -5' |
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| 23406 | 3' | -55.8 | NC_005259.1 | + | 66263 | 0.71 | 0.459675 |
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Target: 5'- gGCCCGCGCcgggCGGCG-GCUCAcgCc- -3' miRNA: 3'- gCGGGCGCGa---GCUGUuCGAGUuaGca -5' |
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| 23406 | 3' | -55.8 | NC_005259.1 | + | 66774 | 0.71 | 0.430875 |
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Target: 5'- cCGCCUGCuGCUCGaacgucGCGAGCgcgUAAUCGa -3' miRNA: 3'- -GCGGGCG-CGAGC------UGUUCGa--GUUAGCa -5' |
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| 23406 | 3' | -55.8 | NC_005259.1 | + | 42149 | 0.71 | 0.430875 |
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Target: 5'- gCGCCCGCGUUggugcacgCGACGAGCagCAcGUCGa -3' miRNA: 3'- -GCGGGCGCGA--------GCUGUUCGa-GU-UAGCa -5' |
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| 23406 | 3' | -55.8 | NC_005259.1 | + | 40563 | 0.72 | 0.394177 |
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Target: 5'- gCGCCCGCGCgguguugcugUCGGCG-GCUCGcaccagcacAUCGUu -3' miRNA: 3'- -GCGGGCGCG----------AGCUGUuCGAGU---------UAGCA- -5' |
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| 23406 | 3' | -55.8 | NC_005259.1 | + | 37581 | 0.74 | 0.327133 |
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Target: 5'- cCGCCCGCGC-CGaucuGCAAGCUgAacGUCGg -3' miRNA: 3'- -GCGGGCGCGaGC----UGUUCGAgU--UAGCa -5' |
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| 23406 | 3' | -55.8 | NC_005259.1 | + | 26647 | 0.74 | 0.289712 |
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Target: 5'- gGCgUGCGCUCGGCAGGUUCGG-CGc -3' miRNA: 3'- gCGgGCGCGAGCUGUUCGAGUUaGCa -5' |
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| 23406 | 3' | -55.8 | NC_005259.1 | + | 10422 | 0.74 | 0.289712 |
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Target: 5'- aGCaCCGUcCUCGGCGAGCUCGAcaUCGa -3' miRNA: 3'- gCG-GGCGcGAGCUGUUCGAGUU--AGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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