Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23409 | 3' | -56.5 | NC_005259.1 | + | 9033 | 0.66 | 0.713077 |
Target: 5'- cGGUGcUCGAucaugugcGGGGCGGUAaccGGCUCGGu -3' miRNA: 3'- -UCGC-AGCU--------CCCUGUCGUcu-UCGAGCC- -5' |
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23409 | 3' | -56.5 | NC_005259.1 | + | 32898 | 0.66 | 0.713077 |
Target: 5'- gGGgG-CGAGGGugAGCcacccGGcccGCUCGGg -3' miRNA: 3'- -UCgCaGCUCCCugUCG-----UCuu-CGAGCC- -5' |
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23409 | 3' | -56.5 | NC_005259.1 | + | 41884 | 0.66 | 0.733781 |
Target: 5'- uGGCGggcCGAuacgcaugcGGGAUgagcGCGGGAGCUCGa -3' miRNA: 3'- -UCGCa--GCU---------CCCUGu---CGUCUUCGAGCc -5' |
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23409 | 3' | -56.5 | NC_005259.1 | + | 34558 | 0.67 | 0.628058 |
Target: 5'- uGcCGUCGGGGccGACGGC-GAGGUucUCGGa -3' miRNA: 3'- uC-GCAGCUCC--CUGUCGuCUUCG--AGCC- -5' |
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23409 | 3' | -56.5 | NC_005259.1 | + | 60738 | 0.67 | 0.628058 |
Target: 5'- cGUGUCGGGGucCAuuugcaguGCAGucGGCUCGGc -3' miRNA: 3'- uCGCAGCUCCcuGU--------CGUCu-UCGAGCC- -5' |
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23409 | 3' | -56.5 | NC_005259.1 | + | 50412 | 0.67 | 0.685722 |
Target: 5'- cGCGcCGGugccuugaccggcucGGG-CAGCGGugcGGGCUCGGg -3' miRNA: 3'- uCGCaGCU---------------CCCuGUCGUC---UUCGAGCC- -5' |
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23409 | 3' | -56.5 | NC_005259.1 | + | 36457 | 0.68 | 0.573647 |
Target: 5'- cGCGUCGAGGGugAGCAccgcccaccacccGAu-CUCGcGg -3' miRNA: 3'- uCGCAGCUCCCugUCGU-------------CUucGAGC-C- -5' |
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23409 | 3' | -56.5 | NC_005259.1 | + | 58949 | 0.68 | 0.585319 |
Target: 5'- cAGCGUCGGccuuGGcCAGCAGGucgggcAGCUCGu -3' miRNA: 3'- -UCGCAGCUc---CCuGUCGUCU------UCGAGCc -5' |
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23409 | 3' | -56.5 | NC_005259.1 | + | 10334 | 0.69 | 0.522457 |
Target: 5'- cGGCGaCGGgcucGGcGGCAGCGGGcucgacaucgGGCUCGGa -3' miRNA: 3'- -UCGCaGCU----CC-CUGUCGUCU----------UCGAGCC- -5' |
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23409 | 3' | -56.5 | NC_005259.1 | + | 27397 | 0.69 | 0.522457 |
Target: 5'- cGGUGUCGGca-GCGGCGGcAGCUCGGu -3' miRNA: 3'- -UCGCAGCUcccUGUCGUCuUCGAGCC- -5' |
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23409 | 3' | -56.5 | NC_005259.1 | + | 59303 | 0.7 | 0.452709 |
Target: 5'- cGCuGUCGuGGcGGCAGCGGcGAGCUCa- -3' miRNA: 3'- uCG-CAGCuCC-CUGUCGUC-UUCGAGcc -5' |
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23409 | 3' | -56.5 | NC_005259.1 | + | 58212 | 0.73 | 0.338184 |
Target: 5'- cGGUGUC-AGGGuCAGUGGccucGAGCUCGGg -3' miRNA: 3'- -UCGCAGcUCCCuGUCGUC----UUCGAGCC- -5' |
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23409 | 3' | -56.5 | NC_005259.1 | + | 68120 | 0.73 | 0.346209 |
Target: 5'- cGgGUCGAGa-GCAGCGGcGAGCUCGGc -3' miRNA: 3'- uCgCAGCUCccUGUCGUC-UUCGAGCC- -5' |
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23409 | 3' | -56.5 | NC_005259.1 | + | 59135 | 0.73 | 0.346209 |
Target: 5'- gAGCGUCGAGcucGGCAGCGGu-GC-CGGg -3' miRNA: 3'- -UCGCAGCUCc--CUGUCGUCuuCGaGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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