Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23416 | 5' | -55 | NC_005259.1 | + | 63288 | 0.66 | 0.793182 |
Target: 5'- uCGAUGCcuucacguGCGAGCAGcggGGUCagGAUGUc -3' miRNA: 3'- -GCUACG--------CGCUCGUCua-CCAG--CUGCAc -5' |
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23416 | 5' | -55 | NC_005259.1 | + | 26442 | 0.67 | 0.7537 |
Target: 5'- cCGAgcGgGCGGGCcGGUGGgcCGAUGUGg -3' miRNA: 3'- -GCUa-CgCGCUCGuCUACCa-GCUGCAC- -5' |
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23416 | 5' | -55 | NC_005259.1 | + | 13781 | 0.67 | 0.7537 |
Target: 5'- uCGAcgGCGaCGAGCAG-UGGUgCGACa-- -3' miRNA: 3'- -GCUa-CGC-GCUCGUCuACCA-GCUGcac -5' |
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23416 | 5' | -55 | NC_005259.1 | + | 18314 | 0.68 | 0.711255 |
Target: 5'- cCGGUGUGCGAcaccgaggccgguGCGuucGUGGUCGACGc- -3' miRNA: 3'- -GCUACGCGCU-------------CGUc--UACCAGCUGCac -5' |
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23416 | 5' | -55 | NC_005259.1 | + | 34122 | 0.68 | 0.687912 |
Target: 5'- cCGG-GCGCGAGCAcguccGGUauccacucuuccgaGGUCGugGUGc -3' miRNA: 3'- -GCUaCGCGCUCGU-----CUA--------------CCAGCugCAC- -5' |
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23416 | 5' | -55 | NC_005259.1 | + | 47156 | 0.68 | 0.673994 |
Target: 5'- -cGUGCGC-AGCAGAUcaccguagcggcgaaGGUCGGCGg- -3' miRNA: 3'- gcUACGCGcUCGUCUA---------------CCAGCUGCac -5' |
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23416 | 5' | -55 | NC_005259.1 | + | 19187 | 0.68 | 0.669698 |
Target: 5'- aCGGUGCccucgacaGCGAGCucGUGGUgcgggcggCGACGUGg -3' miRNA: 3'- -GCUACG--------CGCUCGucUACCA--------GCUGCAC- -5' |
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23416 | 5' | -55 | NC_005259.1 | + | 47704 | 0.69 | 0.604949 |
Target: 5'- gGAUGCGCG-GCAGugccuuGUCGGCGa- -3' miRNA: 3'- gCUACGCGCuCGUCuac---CAGCUGCac -5' |
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23416 | 5' | -55 | NC_005259.1 | + | 65984 | 0.7 | 0.551512 |
Target: 5'- aGgcGCGcCGAGCAGccGcUCGACGUGg -3' miRNA: 3'- gCuaCGC-GCUCGUCuaCcAGCUGCAC- -5' |
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23416 | 5' | -55 | NC_005259.1 | + | 52553 | 0.73 | 0.403162 |
Target: 5'- gGAUGUGCGAGCccGAcaUGGUCGACc-- -3' miRNA: 3'- gCUACGCGCUCGu-CU--ACCAGCUGcac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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