Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23417 | 3' | -54.7 | NC_005259.1 | + | 23301 | 0.66 | 0.797939 |
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Target: 5'- -gGUaUCGGgGUCCggcgcgGUCGGUGUCGCCg -3' miRNA: 3'- caCAaAGUCgCAGG------UAGCCGUGGCGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 22752 | 0.71 | 0.534048 |
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Target: 5'- ------aGGCGUaCCGaaaCGGCACCGCCa -3' miRNA: 3'- cacaaagUCGCA-GGUa--GCCGUGGCGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 22636 | 0.68 | 0.717014 |
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Target: 5'- ---cUUCGccGCGUCgGUCGaCGCCGCCc -3' miRNA: 3'- cacaAAGU--CGCAGgUAGCcGUGGCGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 16681 | 0.66 | 0.788303 |
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Target: 5'- aUGUggUCGGCGaUCC-UgGGCaccgaggugACCGCCg -3' miRNA: 3'- cACAa-AGUCGC-AGGuAgCCG---------UGGCGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 13447 | 0.66 | 0.778508 |
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Target: 5'- ------aGGCGUgaCCGUCGGCGCgGCg -3' miRNA: 3'- cacaaagUCGCA--GGUAGCCGUGgCGg -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 12990 | 0.68 | 0.706413 |
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Target: 5'- cGUGgugcUC-GCGUUCGcacUCGGCuacGCCGCCg -3' miRNA: 3'- -CACaa--AGuCGCAGGU---AGCCG---UGGCGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 12156 | 0.67 | 0.768566 |
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Target: 5'- ----aUCAcCG-CCGaCGGCACCGCCc -3' miRNA: 3'- cacaaAGUcGCaGGUaGCCGUGGCGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 4456 | 0.69 | 0.605827 |
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Target: 5'- aGUGgg-CAGCGUaugggccaaggCCAUCGGCgguggaccauggugAUCGCCa -3' miRNA: 3'- -CACaaaGUCGCA-----------GGUAGCCG--------------UGGCGG- -5' |
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| 23417 | 3' | -54.7 | NC_005259.1 | + | 3130 | 0.71 | 0.492621 |
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Target: 5'- cGUGgg-CAGCGga---CGGCACCGCCc -3' miRNA: 3'- -CACaaaGUCGCagguaGCCGUGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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