miRNA: 3'- -CGccGCCGgGGCG-GGUa---GCGGCUgCG- -5'
miRNA: 3'- -CGCcGcCGGgGCGGg--UAGCGGCUGCG- -5'
miRNA: 3'- cGCCGCCGG---GGCGGguagCGGCUgCG- -5'
miRNA: 3'- -CGCCGc-CGgGGCGGGUagCGGCuGCG- -5'
miRNA: 3'- -CGC-CGc--CGG----GGCGG-GUa-GCGGCUGCG- -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23417 | 5' | -65 | NC_005259.1 | + | 11000 | 0.66 | 0.35764 |
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Target: 5'- cGCaaCGGCaCCGCgCCGgacgCGCCGAgGCa -3' miRNA: 3'- -CGccGCCGgGGCG-GGUa---GCGGCUgCG- -5' |
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| 23417 | 5' | -65 | NC_005259.1 | + | 35267 | 0.66 | 0.35764 |
|
Target: 5'- cGCGcCcGCCgCGCCgggGUCGCCGAUGUg -3' miRNA: 3'- -CGCcGcCGGgGCGGg--UAGCGGCUGCG- -5' |
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| 23417 | 5' | -65 | NC_005259.1 | + | 43732 | 0.66 | 0.35764 |
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Target: 5'- aUGGCGGCCaugCCGCCga--GCCGG-GCa -3' miRNA: 3'- cGCCGCCGG---GGCGGguagCGGCUgCG- -5' |
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| 23417 | 5' | -65 | NC_005259.1 | + | 45337 | 0.66 | 0.35764 |
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Target: 5'- cGUGGCcuGCgCCGCCUGg-GCCGcCGCg -3' miRNA: 3'- -CGCCGc-CGgGGCGGGUagCGGCuGCG- -5' |
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| 23417 | 5' | -65 | NC_005259.1 | + | 37147 | 0.66 | 0.35764 |
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Target: 5'- uGCGcGCauaGCCgucaCCGCCgCGccCGCCGACGCc -3' miRNA: 3'- -CGC-CGc--CGG----GGCGG-GUa-GCGGCUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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