Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23418 | 3' | -50.8 | NC_005259.1 | + | 63077 | 0.77 | 0.433598 |
Target: 5'- cUCAGGGUUcUUGGUGaCGACAUCGGc -3' miRNA: 3'- -AGUUCCAGuAGCCACaGCUGUAGCUc -5' |
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23418 | 3' | -50.8 | NC_005259.1 | + | 23304 | 0.78 | 0.385838 |
Target: 5'- aUCGGGGUCcggcgcgGUCGGUGUCGccgagccggauuACAUCGGGc -3' miRNA: 3'- -AGUUCCAG-------UAGCCACAGC------------UGUAGCUC- -5' |
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23418 | 3' | -50.8 | NC_005259.1 | + | 52765 | 0.78 | 0.377777 |
Target: 5'- aUCGAGcUCGUCGGUGUCGGCAgcgugugccgUCGAc -3' miRNA: 3'- -AGUUCcAGUAGCCACAGCUGU----------AGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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