Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23419 | 3' | -53.3 | NC_005259.1 | + | 67608 | 0.71 | 0.562716 |
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Target: 5'- aCGUGUUCGA-GCCACCGACGAaaaCCa- -3' miRNA: 3'- -GUACGAGUUcCGGUGGUUGCUa--GGac -5' |
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| 23419 | 3' | -53.3 | NC_005259.1 | + | 50207 | 0.71 | 0.551826 |
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Target: 5'- --cGCUCAAGcGCCGCCucgucaaucuCGAUCCg- -3' miRNA: 3'- guaCGAGUUC-CGGUGGuu--------GCUAGGac -5' |
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| 23419 | 3' | -53.3 | NC_005259.1 | + | 57966 | 0.72 | 0.508984 |
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Target: 5'- cCAUGgUCGAGGUCGCCGG-GAUCgUGa -3' miRNA: 3'- -GUACgAGUUCCGGUGGUUgCUAGgAC- -5' |
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| 23419 | 3' | -53.3 | NC_005259.1 | + | 23348 | 0.72 | 0.477792 |
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Target: 5'- --gGCUCGAGGCUGCCuGCGAggCCg- -3' miRNA: 3'- guaCGAGUUCCGGUGGuUGCUa-GGac -5' |
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| 23419 | 3' | -53.3 | NC_005259.1 | + | 63550 | 0.73 | 0.468619 |
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Target: 5'- gGUGCUCGGcGGCCACCGacgcgACGAgcgggauauacgccgCCUGg -3' miRNA: 3'- gUACGAGUU-CCGGUGGU-----UGCUa--------------GGAC- -5' |
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| 23419 | 3' | -53.3 | NC_005259.1 | + | 65413 | 0.73 | 0.428027 |
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Target: 5'- uCAUGCUCAcGGUgGCCAaGCGAUggCCUGa -3' miRNA: 3'- -GUACGAGUuCCGgUGGU-UGCUA--GGAC- -5' |
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| 23419 | 3' | -53.3 | NC_005259.1 | + | 17846 | 0.74 | 0.418445 |
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Target: 5'- --gGC-CGAGGCCACCccguggucAGCGAUCCUu -3' miRNA: 3'- guaCGaGUUCCGGUGG--------UUGCUAGGAc -5' |
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| 23419 | 3' | -53.3 | NC_005259.1 | + | 42778 | 0.76 | 0.322184 |
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Target: 5'- --gGuCUCGGGGCCGCCAcCGAUCaCUGu -3' miRNA: 3'- guaC-GAGUUCCGGUGGUuGCUAG-GAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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