Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23422 | 3' | -55.7 | NC_005259.1 | + | 44262 | 0.66 | 0.711526 |
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Target: 5'- -aAGUCGGCGACauc--GAgGCCGAAc -3' miRNA: 3'- caUCAGCCGCUGacaacCUgCGGCUU- -5' |
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| 23422 | 3' | -55.7 | NC_005259.1 | + | 27174 | 0.66 | 0.711526 |
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Target: 5'- --cGUCGGCGAUgagcagGcUGcGACGCUGGAa -3' miRNA: 3'- cauCAGCCGCUGa-----CaAC-CUGCGGCUU- -5' |
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| 23422 | 3' | -55.7 | NC_005259.1 | + | 55329 | 0.66 | 0.700864 |
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Target: 5'- gGUGGUCuuGGCGGCagcgGUgGcGAUGCCGAAc -3' miRNA: 3'- -CAUCAG--CCGCUGa---CAaC-CUGCGGCUU- -5' |
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| 23422 | 3' | -55.7 | NC_005259.1 | + | 17359 | 0.67 | 0.679356 |
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Target: 5'- -aGGUCGGCGGCUc---GGCGCUGGu -3' miRNA: 3'- caUCAGCCGCUGAcaacCUGCGGCUu -5' |
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| 23422 | 3' | -55.7 | NC_005259.1 | + | 60761 | 0.68 | 0.614101 |
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Target: 5'- -cAGUCGGCucGGCgagGUUGGGCuGCgCGAAc -3' miRNA: 3'- caUCAGCCG--CUGa--CAACCUG-CG-GCUU- -5' |
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| 23422 | 3' | -55.7 | NC_005259.1 | + | 23641 | 0.68 | 0.602137 |
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Target: 5'- uGUGGUuucggcccgccgaCGGCGACgGcUGGAcCGCCGAc -3' miRNA: 3'- -CAUCA-------------GCCGCUGaCaACCU-GCGGCUu -5' |
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| 23422 | 3' | -55.7 | NC_005259.1 | + | 28235 | 0.7 | 0.517759 |
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Target: 5'- --cGUCGGCGGCgg--GGugGUCGAu -3' miRNA: 3'- cauCAGCCGCUGacaaCCugCGGCUu -5' |
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| 23422 | 3' | -55.7 | NC_005259.1 | + | 16682 | 0.72 | 0.400165 |
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Target: 5'- uGUGGUCGGCGAUcc-UGGGCaCCGAGg -3' miRNA: 3'- -CAUCAGCCGCUGacaACCUGcGGCUU- -5' |
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| 23422 | 3' | -55.7 | NC_005259.1 | + | 39841 | 0.74 | 0.279432 |
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Target: 5'- uUGGUCGGCGACa---GGAUGCCGGu -3' miRNA: 3'- cAUCAGCCGCUGacaaCCUGCGGCUu -5' |
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| 23422 | 3' | -55.7 | NC_005259.1 | + | 64167 | 0.81 | 0.109688 |
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Target: 5'- cGUAGUCGGCGACUGUcUGuGCGUCGGGa -3' miRNA: 3'- -CAUCAGCCGCUGACA-ACcUGCGGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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