Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23424 | 3' | -59.1 | NC_005259.1 | + | 26814 | 0.74 | 0.181887 |
Target: 5'- uUGGCCGGuACCGGCggUGGUGCcgggGCGGUc -3' miRNA: 3'- -ACUGGCC-UGGCUGa-GCCACGa---CGCUA- -5' |
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23424 | 3' | -59.1 | NC_005259.1 | + | 56032 | 0.71 | 0.307702 |
Target: 5'- gGGCgGGACUucCUCGGgcgGCUGCGGg -3' miRNA: 3'- aCUGgCCUGGcuGAGCCa--CGACGCUa -5' |
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23424 | 3' | -59.1 | NC_005259.1 | + | 4682 | 0.7 | 0.330318 |
Target: 5'- cUGGCuCGGGCCGacaGCUCGGccccgUGgUGCGAUg -3' miRNA: 3'- -ACUG-GCCUGGC---UGAGCC-----ACgACGCUA- -5' |
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23424 | 3' | -59.1 | NC_005259.1 | + | 50414 | 0.69 | 0.369835 |
Target: 5'- -cGCCGGugccuugACCGGCUCGG-GCaGCGGUg -3' miRNA: 3'- acUGGCC-------UGGCUGAGCCaCGaCGCUA- -5' |
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23424 | 3' | -59.1 | NC_005259.1 | + | 20993 | 0.69 | 0.37914 |
Target: 5'- cGugUGGGCCuACUCGG-GCgGCGAc -3' miRNA: 3'- aCugGCCUGGcUGAGCCaCGaCGCUa -5' |
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23424 | 3' | -59.1 | NC_005259.1 | + | 62736 | 0.69 | 0.387734 |
Target: 5'- cGACCGGACCGGCggcaguacgCGGUGgUacucGUGGg -3' miRNA: 3'- aCUGGCCUGGCUGa--------GCCACgA----CGCUa -5' |
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23424 | 3' | -59.1 | NC_005259.1 | + | 5073 | 0.69 | 0.405301 |
Target: 5'- gGGCCGGGUCGACgggcCGGUGUcaccgGCGAUc -3' miRNA: 3'- aCUGGCCUGGCUGa---GCCACGa----CGCUA- -5' |
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23424 | 3' | -59.1 | NC_005259.1 | + | 66657 | 0.69 | 0.414271 |
Target: 5'- -uGCCGG-UCGg--CGGUGCUGCGAUg -3' miRNA: 3'- acUGGCCuGGCugaGCCACGACGCUA- -5' |
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23424 | 3' | -59.1 | NC_005259.1 | + | 46986 | 0.68 | 0.423361 |
Target: 5'- aUGAUCGGGCCGAg--GGUGCgccacGCGAg -3' miRNA: 3'- -ACUGGCCUGGCUgagCCACGa----CGCUa -5' |
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23424 | 3' | -59.1 | NC_005259.1 | + | 33911 | 0.68 | 0.423361 |
Target: 5'- gGGCCGaGACCGGCcaCGGUGCgaGCa-- -3' miRNA: 3'- aCUGGC-CUGGCUGa-GCCACGa-CGcua -5' |
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23424 | 3' | -59.1 | NC_005259.1 | + | 7347 | 0.68 | 0.451333 |
Target: 5'- aGGCCGGACaGACguucGUGCUGUGGc -3' miRNA: 3'- aCUGGCCUGgCUGagc-CACGACGCUa -5' |
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23424 | 3' | -59.1 | NC_005259.1 | + | 29001 | 0.67 | 0.500084 |
Target: 5'- cGGCCacgaGAUCGGCgaaugCGGUGuCUGCGGUc -3' miRNA: 3'- aCUGGc---CUGGCUGa----GCCAC-GACGCUA- -5' |
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23424 | 3' | -59.1 | NC_005259.1 | + | 23719 | 0.66 | 0.551027 |
Target: 5'- -cGCCGGACgG-UUCGGUGCUcacgugggacgGCGAc -3' miRNA: 3'- acUGGCCUGgCuGAGCCACGA-----------CGCUa -5' |
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23424 | 3' | -59.1 | NC_005259.1 | + | 16220 | 0.66 | 0.561418 |
Target: 5'- cUGAUCagguGGGCCGAacgcacccCGGUGCUGCGc- -3' miRNA: 3'- -ACUGG----CCUGGCUga------GCCACGACGCua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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