Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23429 | 3' | -53.1 | NC_005259.1 | + | 19380 | 0.71 | 0.602349 |
Target: 5'- gCUGAUCACCGGC--CC-CAAcggcAGCGUCg -3' miRNA: 3'- -GGCUAGUGGCCGcuGGaGUU----UCGUAG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 57994 | 0.66 | 0.894774 |
Target: 5'- cCCGGUCgaGCCaGCGACCauguuGAGgAUCg -3' miRNA: 3'- -GGCUAG--UGGcCGCUGGagu--UUCgUAG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 58341 | 0.69 | 0.721763 |
Target: 5'- -aGAUCAggUCGGUGGCCUC-GGGCAcgUCg -3' miRNA: 3'- ggCUAGU--GGCCGCUGGAGuUUCGU--AG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 52570 | 0.7 | 0.70042 |
Target: 5'- aUGGUCgACCGGacCGGCCU--GAGCGUCg -3' miRNA: 3'- gGCUAG-UGGCC--GCUGGAguUUCGUAG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 42913 | 0.7 | 0.70042 |
Target: 5'- -aGAUCGuuGGUGGCCUUG-AGCAUg -3' miRNA: 3'- ggCUAGUggCCGCUGGAGUuUCGUAg -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 42763 | 0.7 | 0.678813 |
Target: 5'- cUCGAUCuGCuCGGCGGUCUCGGGGCcgCc -3' miRNA: 3'- -GGCUAG-UG-GCCGCUGGAGUUUCGuaG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 67737 | 0.7 | 0.667938 |
Target: 5'- uCCGAU-ACCGGCGGCCacuAGGCcgCc -3' miRNA: 3'- -GGCUAgUGGCCGCUGGaguUUCGuaG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 6516 | 0.7 | 0.657031 |
Target: 5'- aCCGA-CGCCGuGCGcgcgacaaccgGCCUCGGGGCGa- -3' miRNA: 3'- -GGCUaGUGGC-CGC-----------UGGAGUUUCGUag -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 67777 | 0.71 | 0.635157 |
Target: 5'- aCCGAcCACgCGGUGACCUUguuGGCAc- -3' miRNA: 3'- -GGCUaGUG-GCCGCUGGAGuu-UCGUag -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 3164 | 0.71 | 0.624211 |
Target: 5'- aCGGUgAugcCCGGCGAUgUCAAGGuCGUCg -3' miRNA: 3'- gGCUAgU---GGCCGCUGgAGUUUC-GUAG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 3704 | 0.71 | 0.624211 |
Target: 5'- uUGAg-ACCGGCGACCUCuuccGUAUCc -3' miRNA: 3'- gGCUagUGGCCGCUGGAGuuu-CGUAG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 53014 | 0.79 | 0.250953 |
Target: 5'- aCUGGgcgCugCGGUGGCgUCGAGGCAUCg -3' miRNA: 3'- -GGCUa--GugGCCGCUGgAGUUUCGUAG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 1226 | 0.72 | 0.558996 |
Target: 5'- aCCGAUCACgaggaCGGCugcaagaccGGCCUC--GGCAUCa -3' miRNA: 3'- -GGCUAGUG-----GCCG---------CUGGAGuuUCGUAG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 5088 | 0.72 | 0.548283 |
Target: 5'- gCCGGugUCACCGGCGAUCUgcgUAGAccGCGUUg -3' miRNA: 3'- -GGCU--AGUGGCCGCUGGA---GUUU--CGUAG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 21033 | 0.72 | 0.537635 |
Target: 5'- aCCGAUgACCGGCGcGCgUUCAAGGUcgCc -3' miRNA: 3'- -GGCUAgUGGCCGC-UG-GAGUUUCGuaG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 39563 | 0.74 | 0.445765 |
Target: 5'- gCCGGUCGuCCGGCccGACCUCGAuGCu-- -3' miRNA: 3'- -GGCUAGU-GGCCG--CUGGAGUUuCGuag -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 68746 | 0.74 | 0.442851 |
Target: 5'- gCCGAgcUCGaccCCGGCGACCUCGccaucgguguacuuGAGCAggUCg -3' miRNA: 3'- -GGCU--AGU---GGCCGCUGGAGU--------------UUCGU--AG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 1531 | 0.74 | 0.426542 |
Target: 5'- aCGAUCACCGGC-AUCaUCGGGGCcgCg -3' miRNA: 3'- gGCUAGUGGCCGcUGG-AGUUUCGuaG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 9536 | 0.75 | 0.398652 |
Target: 5'- gCCgGGUCACCGGagagcugccCGGCCUCGAccGCGUCg -3' miRNA: 3'- -GG-CUAGUGGCC---------GCUGGAGUUu-CGUAG- -5' |
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23429 | 3' | -53.1 | NC_005259.1 | + | 8365 | 0.77 | 0.306924 |
Target: 5'- aUCGcUCGgCGGCGGCCUC--GGCAUCg -3' miRNA: 3'- -GGCuAGUgGCCGCUGGAGuuUCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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