Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23435 | 3' | -53.7 | NC_005259.1 | + | 14850 | 0.72 | 0.491982 |
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Target: 5'- gGACACgGuCGUGCUCGcgagguuCGCGCUGc -3' miRNA: 3'- gUUGUGgCuGCACGAGUu------GCGCGACu -5' |
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| 23435 | 3' | -53.7 | NC_005259.1 | + | 57011 | 0.72 | 0.502283 |
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Target: 5'- gAGCACCucaGCGcgGCUCGGCGCGgUGGg -3' miRNA: 3'- gUUGUGGc--UGCa-CGAGUUGCGCgACU- -5' |
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| 23435 | 3' | -53.7 | NC_005259.1 | + | 13463 | 0.72 | 0.53372 |
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Target: 5'- gCGGCGCUG--GUGCUCAcCGCGCUGc -3' miRNA: 3'- -GUUGUGGCugCACGAGUuGCGCGACu -5' |
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| 23435 | 3' | -53.7 | NC_005259.1 | + | 44038 | 0.69 | 0.661917 |
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Target: 5'- gAACACCGGCcuugacgaccgGCUCGAgUGCGCUGu -3' miRNA: 3'- gUUGUGGCUGca---------CGAGUU-GCGCGACu -5' |
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| 23435 | 3' | -53.7 | NC_005259.1 | + | 640 | 0.69 | 0.664102 |
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Target: 5'- aCAACGCCaccgccgagGACGUGCUCGAgGgcaUGCUGu -3' miRNA: 3'- -GUUGUGG---------CUGCACGAGUUgC---GCGACu -5' |
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| 23435 | 3' | -53.7 | NC_005259.1 | + | 65787 | 0.69 | 0.664102 |
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Target: 5'- -uGCGCC-AUG-GcCUCGACGCGCUGGu -3' miRNA: 3'- guUGUGGcUGCaC-GAGUUGCGCGACU- -5' |
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| 23435 | 3' | -53.7 | NC_005259.1 | + | 5192 | 0.69 | 0.664102 |
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Target: 5'- cCGACGCCGcCGccaaGCUCAcCGCGCUc- -3' miRNA: 3'- -GUUGUGGCuGCa---CGAGUuGCGCGAcu -5' |
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| 23435 | 3' | -53.7 | NC_005259.1 | + | 58745 | 0.69 | 0.664102 |
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Target: 5'- cCGGCACCGGCGaaGUUCGGCGaacgaCGCUGc -3' miRNA: 3'- -GUUGUGGCUGCa-CGAGUUGC-----GCGACu -5' |
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| 23435 | 3' | -53.7 | NC_005259.1 | + | 50682 | 0.69 | 0.69668 |
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Target: 5'- uCggUGCCGACGaggcgcUGCUCGucguCGCGCUuGAg -3' miRNA: 3'- -GuuGUGGCUGC------ACGAGUu---GCGCGA-CU- -5' |
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| 23435 | 3' | -53.7 | NC_005259.1 | + | 12986 | 0.66 | 0.861909 |
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Target: 5'- --cCACCGugGUGCUC-GCGUucGCa-- -3' miRNA: 3'- guuGUGGCugCACGAGuUGCG--CGacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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