miRNA display CGI


Results 1 - 15 of 15 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23436 5' -52.6 NC_005259.1 + 778 0.66 0.898053
Target:  5'- -cGCCgagGCGGcugccCUCgguuGGGUGGCCAagggGCCg -3'
miRNA:   3'- guUGGa--UGUCa----GAG----UCCACCGGU----UGG- -5'
23436 5' -52.6 NC_005259.1 + 57372 0.66 0.898053
Target:  5'- -cACCguggGCAGUCUCGGGaaGGUUcuuGCCc -3'
miRNA:   3'- guUGGa---UGUCAGAGUCCa-CCGGu--UGG- -5'
23436 5' -52.6 NC_005259.1 + 52583 0.66 0.875835
Target:  5'- cCGGCCUGagcGUCgccgagCAccagcgccguacGGUGGCCAACUa -3'
miRNA:   3'- -GUUGGAUgu-CAGa-----GU------------CCACCGGUUGG- -5'
23436 5' -52.6 NC_005259.1 + 11399 0.66 0.867912
Target:  5'- -uGCCUcaaGCGGUUUCGGcucGUGGCCggUg -3'
miRNA:   3'- guUGGA---UGUCAGAGUC---CACCGGuuGg -5'
23436 5' -52.6 NC_005259.1 + 19910 0.66 0.867912
Target:  5'- aCAACC-ACGGUUUCGGcaagGGCCAGa- -3'
miRNA:   3'- -GUUGGaUGUCAGAGUCca--CCGGUUgg -5'
23436 5' -52.6 NC_005259.1 + 46604 0.67 0.851328
Target:  5'- gCAGCgCgucguugGCGGUCUU-GGUGGCCuGACUg -3'
miRNA:   3'- -GUUG-Ga------UGUCAGAGuCCACCGG-UUGG- -5'
23436 5' -52.6 NC_005259.1 + 23624 0.67 0.842683
Target:  5'- gCAGCCagACGG-CUCGGuGUGGUuuCGGCCc -3'
miRNA:   3'- -GUUGGa-UGUCaGAGUC-CACCG--GUUGG- -5'
23436 5' -52.6 NC_005259.1 + 51420 0.67 0.82473
Target:  5'- gGGCCgguugaGGUCUUGGGcgGGCCAcgggGCCu -3'
miRNA:   3'- gUUGGaug---UCAGAGUCCa-CCGGU----UGG- -5'
23436 5' -52.6 NC_005259.1 + 57317 0.67 0.82473
Target:  5'- -cACCgGCGGUgUCGGuGUGGUCAugUg -3'
miRNA:   3'- guUGGaUGUCAgAGUC-CACCGGUugG- -5'
23436 5' -52.6 NC_005259.1 + 4112 0.69 0.713653
Target:  5'- aAACCUcgGCGGcCUCGacGUGGCCAagACCg -3'
miRNA:   3'- gUUGGA--UGUCaGAGUc-CACCGGU--UGG- -5'
23436 5' -52.6 NC_005259.1 + 42764 0.7 0.702835
Target:  5'- uCGAUCUgcucgGCGGUCUCGGGgccGCC-ACCg -3'
miRNA:   3'- -GUUGGA-----UGUCAGAGUCCac-CGGuUGG- -5'
23436 5' -52.6 NC_005259.1 + 54693 0.7 0.691947
Target:  5'- aGACCgu--GUCgaggCGGGUGGCCucAGCCu -3'
miRNA:   3'- gUUGGauguCAGa---GUCCACCGG--UUGG- -5'
23436 5' -52.6 NC_005259.1 + 15551 0.7 0.670008
Target:  5'- uGGCCggUGCcaccCUCAGGUGGCCAcCCu -3'
miRNA:   3'- gUUGG--AUGuca-GAGUCCACCGGUuGG- -5'
23436 5' -52.6 NC_005259.1 + 32435 0.73 0.495868
Target:  5'- gCAGCU--CGGUCUCAGGguccaucaucuUGGCCAGCa -3'
miRNA:   3'- -GUUGGauGUCAGAGUCC-----------ACCGGUUGg -5'
23436 5' -52.6 NC_005259.1 + 9971 0.77 0.297568
Target:  5'- cCGACCaccgcacCGGUCg-AGGUGGCCAGCCg -3'
miRNA:   3'- -GUUGGau-----GUCAGagUCCACCGGUUGG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.