Results 21 - 40 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23437 | 3' | -60 | NC_005259.1 | + | 14285 | 0.68 | 0.392167 |
Target: 5'- -cCGCCugcccaaccaccgAugUCGGUGcguaUgGCCGCCCUCg -3' miRNA: 3'- aaGCGG-------------UugAGCCGC----AgUGGCGGGAG- -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 13002 | 0.68 | 0.393041 |
Target: 5'- gUUCGC--ACUCGGCuacGcCGCCGCCCg- -3' miRNA: 3'- -AAGCGguUGAGCCG---CaGUGGCGGGag -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 45377 | 0.68 | 0.393041 |
Target: 5'- uUUUGCCcgaggaauuuCUCGGgGUCGCCGCCg-- -3' miRNA: 3'- -AAGCGGuu--------GAGCCgCAGUGGCGGgag -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 9697 | 0.68 | 0.401851 |
Target: 5'- gUCGCCGcucACUgGGC---ACCGUCCUCg -3' miRNA: 3'- aAGCGGU---UGAgCCGcagUGGCGGGAG- -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 40663 | 0.68 | 0.409886 |
Target: 5'- cUCGCCGAgcagcgcgagcgcCUUGGUGcUCACCGgCCCg- -3' miRNA: 3'- aAGCGGUU-------------GAGCCGC-AGUGGC-GGGag -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 38640 | 0.68 | 0.410785 |
Target: 5'- -aCGCCGAgUUGGCGaacUCGCCGaaaucgaccaCCUCg -3' miRNA: 3'- aaGCGGUUgAGCCGC---AGUGGCg---------GGAG- -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 19058 | 0.67 | 0.419842 |
Target: 5'- -gCGCCaccgAugUCGGUGUCGCgGUCCg- -3' miRNA: 3'- aaGCGG----UugAGCCGCAGUGgCGGGag -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 12331 | 0.67 | 0.429018 |
Target: 5'- -cCGCCAGCUCa-CG-CACCGCcgCCUCg -3' miRNA: 3'- aaGCGGUUGAGccGCaGUGGCG--GGAG- -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 12160 | 0.67 | 0.44772 |
Target: 5'- -cCGCCGA--CGGC---ACCGCCCUCg -3' miRNA: 3'- aaGCGGUUgaGCCGcagUGGCGGGAG- -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 68455 | 0.67 | 0.44772 |
Target: 5'- cUCGaCCAGCUUGGU--CACCGCCgUg -3' miRNA: 3'- aAGC-GGUUGAGCCGcaGUGGCGGgAg -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 21478 | 0.67 | 0.44772 |
Target: 5'- --aGCCGA-UCcGCGaacugCACCGCCCUCg -3' miRNA: 3'- aagCGGUUgAGcCGCa----GUGGCGGGAG- -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 17361 | 0.67 | 0.44772 |
Target: 5'- gUCGgCGGCUCGGCGcuggucguggUCgacugcgacacuGCCGCCCa- -3' miRNA: 3'- aAGCgGUUGAGCCGC----------AG------------UGGCGGGag -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 52370 | 0.67 | 0.466865 |
Target: 5'- aUCGCCGACUCaaGCGgCugCGCagccugCCUCg -3' miRNA: 3'- aAGCGGUUGAGc-CGCaGugGCG------GGAG- -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 29221 | 0.66 | 0.476595 |
Target: 5'- gUUCGCCAGcCUCGGCGcguuccugaUCAucCUGUCCaUCg -3' miRNA: 3'- -AAGCGGUU-GAGCCGC---------AGU--GGCGGG-AG- -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 61150 | 0.66 | 0.476595 |
Target: 5'- -gUGCCGACUcaaggacaccgaCGGUGUaCGCCucgguaugGCCCUCg -3' miRNA: 3'- aaGCGGUUGA------------GCCGCA-GUGG--------CGGGAG- -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 48528 | 0.66 | 0.486425 |
Target: 5'- -cUGCCGACuucUCGGCGcugUCGCCGgUCUUg -3' miRNA: 3'- aaGCGGUUG---AGCCGC---AGUGGCgGGAG- -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 46230 | 0.66 | 0.486425 |
Target: 5'- aUUgGUCAGCUCGGCu--GCCGCCUg- -3' miRNA: 3'- -AAgCGGUUGAGCCGcagUGGCGGGag -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 57125 | 0.66 | 0.49635 |
Target: 5'- cUCGCCca-UCGGCGggACCgagcaguagugGCCCUCc -3' miRNA: 3'- aAGCGGuugAGCCGCagUGG-----------CGGGAG- -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 68261 | 0.66 | 0.516465 |
Target: 5'- --aGCCuugcGCUCGuCGUaggCACCGUCCUCg -3' miRNA: 3'- aagCGGu---UGAGCcGCA---GUGGCGGGAG- -5' |
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23437 | 3' | -60 | NC_005259.1 | + | 3260 | 0.66 | 0.516465 |
Target: 5'- gUCGgCAuccuGCUCGGgugguuuccCGUCGCCGCCUc- -3' miRNA: 3'- aAGCgGU----UGAGCC---------GCAGUGGCGGGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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