Results 21 - 40 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23437 | 5' | -54.8 | NC_005259.1 | + | 65544 | 0.66 | 0.837097 |
Target: 5'- aCCGAGGuacucgacaagaUCGGcccauaGACACCGaGGgaCGGGCg -3' miRNA: 3'- -GGUUCC------------AGUCa-----CUGUGGC-CUa-GCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 66937 | 0.66 | 0.837097 |
Target: 5'- aCCGGGGUgAGcggGACACCGG--CGaGCu -3' miRNA: 3'- -GGUUCCAgUCa--CUGUGGCCuaGCcCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 59064 | 0.7 | 0.583392 |
Target: 5'- -aGGGGUCAGgcucuCACCGGccuuguggCGGGCa -3' miRNA: 3'- ggUUCCAGUCacu--GUGGCCua------GCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 18153 | 0.77 | 0.257554 |
Target: 5'- aCGAGaUCGGgcUGACGCCGGAUCuGGCa -3' miRNA: 3'- gGUUCcAGUC--ACUGUGGCCUAGcCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 7598 | 0.76 | 0.31416 |
Target: 5'- cUCAAGGUCGGUGACACCcucacccacgaGGGCc -3' miRNA: 3'- -GGUUCCAGUCACUGUGGccuag------CCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 23311 | 0.74 | 0.377966 |
Target: 5'- uCCGGcgcGGUCGGUGucgccgaGCCGGAuuacaUCGGGCu -3' miRNA: 3'- -GGUU---CCAGUCACug-----UGGCCU-----AGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 63658 | 0.74 | 0.395443 |
Target: 5'- ---uGGcCGG-GACGCCGGAcaccUCGGGCa -3' miRNA: 3'- gguuCCaGUCaCUGUGGCCU----AGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 45850 | 0.73 | 0.413434 |
Target: 5'- aCCGGGGcCGGUGGgACCGGGcaugcCGGGg -3' miRNA: 3'- -GGUUCCaGUCACUgUGGCCUa----GCCCg -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 64034 | 0.72 | 0.487114 |
Target: 5'- gCGAGGcCGGUGACcggaucuuucucuuGCCGGAUCagcacGGCg -3' miRNA: 3'- gGUUCCaGUCACUG--------------UGGCCUAGc----CCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 57470 | 0.72 | 0.500171 |
Target: 5'- --cAGGcCGGUGGCACCGGAcacgUCGaGCg -3' miRNA: 3'- gguUCCaGUCACUGUGGCCU----AGCcCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 58549 | 0.71 | 0.551701 |
Target: 5'- gUCGAGGUuacggcucgggCGGUuGgGCCGGAUCGcGGCg -3' miRNA: 3'- -GGUUCCA-----------GUCAcUgUGGCCUAGC-CCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 38627 | 0.71 | 0.551701 |
Target: 5'- aUCGAGGgCGGUGACGCCGaGUUGG-Cg -3' miRNA: 3'- -GGUUCCaGUCACUGUGGCcUAGCCcG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 52408 | 0.7 | 0.572779 |
Target: 5'- aUCGAGGUguG-GGCGCUcGAcUCGGGCg -3' miRNA: 3'- -GGUUCCAguCaCUGUGGcCU-AGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 58216 | 0.8 | 0.170396 |
Target: 5'- gUCAGGGUCAGUGGCcUCGaGcUCGGGCu -3' miRNA: 3'- -GGUUCCAGUCACUGuGGC-CuAGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 34152 | 0.7 | 0.583392 |
Target: 5'- uCCGAGGUC-GUGGugccccaccagUGCCGGAacacacCGGGCa -3' miRNA: 3'- -GGUUCCAGuCACU-----------GUGGCCUa-----GCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 27478 | 0.7 | 0.609003 |
Target: 5'- gCCGGGGUCGGUGuggccaccgccccacGCGCCGaGGUCGa-- -3' miRNA: 3'- -GGUUCCAGUCAC---------------UGUGGC-CUAGCccg -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 24369 | 0.7 | 0.615427 |
Target: 5'- aCCGAGGUCuGGUGGCgcuACCGGAguacgUCGaacGCa -3' miRNA: 3'- -GGUUCCAG-UCACUG---UGGCCU-----AGCc--CG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 9192 | 0.69 | 0.668973 |
Target: 5'- gUCGAGGUCGucauacGGgGCCGGAUCGaGCa -3' miRNA: 3'- -GGUUCCAGUca----CUgUGGCCUAGCcCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 9691 | 0.69 | 0.679622 |
Target: 5'- gUCGAGGUCGccgcucacugGGCACCGuccUCGGGCc -3' miRNA: 3'- -GGUUCCAGUca--------CUGUGGCcu-AGCCCG- -5' |
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23437 | 5' | -54.8 | NC_005259.1 | + | 25683 | 0.68 | 0.685992 |
Target: 5'- gUCGAGGcCGGUgucccaccccaaagGACACCGGccucgcuGUUGGGUa -3' miRNA: 3'- -GGUUCCaGUCA--------------CUGUGGCC-------UAGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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