Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23438 | 3' | -54.1 | NC_005259.1 | + | 13455 | 0.66 | 0.858553 |
Target: 5'- ---cCGUCGGCgCGGCGCuGGUGc-- -3' miRNA: 3'- agauGUAGCCGaGCUGCGuCCACacc -5' |
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23438 | 3' | -54.1 | NC_005259.1 | + | 59136 | 0.67 | 0.796355 |
Target: 5'- --aGCGUCGaGCUCGGCaGC-GGUGccgGGg -3' miRNA: 3'- agaUGUAGC-CGAGCUG-CGuCCACa--CC- -5' |
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23438 | 3' | -54.1 | NC_005259.1 | + | 65095 | 0.67 | 0.786727 |
Target: 5'- --aGC-UCGGCUCGugGCAcGGUcaGUGc -3' miRNA: 3'- agaUGuAGCCGAGCugCGU-CCA--CACc -5' |
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23438 | 3' | -54.1 | NC_005259.1 | + | 45996 | 0.67 | 0.786727 |
Target: 5'- -gUACAUcugCGGgUCGACGCGaacGGUGaUGGu -3' miRNA: 3'- agAUGUA---GCCgAGCUGCGU---CCAC-ACC- -5' |
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23438 | 3' | -54.1 | NC_005259.1 | + | 19956 | 0.68 | 0.73646 |
Target: 5'- gUCcGCAacggCGGCUCGGC---GGUGUGGg -3' miRNA: 3'- -AGaUGUa---GCCGAGCUGcguCCACACC- -5' |
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23438 | 3' | -54.1 | NC_005259.1 | + | 62991 | 0.68 | 0.725013 |
Target: 5'- --cGCGUCGaCUCGAUggcauugGCAGGcUGUGGg -3' miRNA: 3'- agaUGUAGCcGAGCUG-------CGUCC-ACACC- -5' |
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23438 | 3' | -54.1 | NC_005259.1 | + | 3342 | 0.69 | 0.68365 |
Target: 5'- cUCUGCGugcUCGGCacgacUCGACGUuaAGGUGUu- -3' miRNA: 3'- -AGAUGU---AGCCG-----AGCUGCG--UCCACAcc -5' |
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23438 | 3' | -54.1 | NC_005259.1 | + | 52635 | 0.69 | 0.672902 |
Target: 5'- gCUGCGUCGGCUgUGGgGCgAGGUGa-- -3' miRNA: 3'- aGAUGUAGCCGA-GCUgCG-UCCACacc -5' |
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23438 | 3' | -54.1 | NC_005259.1 | + | 11084 | 0.69 | 0.672902 |
Target: 5'- ---cCGUaCGGCUCGAccuCGCAGGUGUu- -3' miRNA: 3'- agauGUA-GCCGAGCU---GCGUCCACAcc -5' |
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23438 | 3' | -54.1 | NC_005259.1 | + | 11406 | 0.7 | 0.640475 |
Target: 5'- --aGCGguuUCGGCUCGugGCcGGUGcaacgGGg -3' miRNA: 3'- agaUGU---AGCCGAGCugCGuCCACa----CC- -5' |
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23438 | 3' | -54.1 | NC_005259.1 | + | 60758 | 0.71 | 0.564974 |
Target: 5'- -gUGCAgUCGGCUCGGCG-AGGU-UGGg -3' miRNA: 3'- agAUGU-AGCCGAGCUGCgUCCAcACC- -5' |
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23438 | 3' | -54.1 | NC_005259.1 | + | 17355 | 0.73 | 0.433072 |
Target: 5'- gUCgagguCggCGGCUCGGCGCuGGUcGUGGu -3' miRNA: 3'- -AGau---GuaGCCGAGCUGCGuCCA-CACC- -5' |
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23438 | 3' | -54.1 | NC_005259.1 | + | 57017 | 0.74 | 0.423655 |
Target: 5'- cUCaGCG-CGGCUCGGCGC-GGUG-GGg -3' miRNA: 3'- -AGaUGUaGCCGAGCUGCGuCCACaCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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