Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23440 | 3' | -54.2 | NC_005259.1 | + | 50111 | 0.67 | 0.770562 |
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Target: 5'- aGGUUGcccguacGCA-CGGGCaccGCCACACGCGAu -3' miRNA: 3'- gUCAGC-------UGUaGCCUG---UGGUGUGCGUU- -5' |
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| 23440 | 3' | -54.2 | NC_005259.1 | + | 21177 | 0.66 | 0.791443 |
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Target: 5'- gCAGUCGGguCAUCGGcgcgaGCACCAaGCuGCAAg -3' miRNA: 3'- -GUCAGCU--GUAGCC-----UGUGGUgUG-CGUU- -5' |
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| 23440 | 3' | -54.2 | NC_005259.1 | + | 46331 | 0.66 | 0.791443 |
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Target: 5'- gGGUCG-CGUCGcGCACCGCuuGUAGg -3' miRNA: 3'- gUCAGCuGUAGCcUGUGGUGugCGUU- -5' |
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| 23440 | 3' | -54.2 | NC_005259.1 | + | 7420 | 0.66 | 0.81065 |
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Target: 5'- -cGUCGcCGUCGGcggugcccGCGCCGCAUgGCAGc -3' miRNA: 3'- guCAGCuGUAGCC--------UGUGGUGUG-CGUU- -5' |
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| 23440 | 3' | -54.2 | NC_005259.1 | + | 63671 | 0.66 | 0.819979 |
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Target: 5'- ----gGACAccUCGGGCACCgGCGCGCc- -3' miRNA: 3'- gucagCUGU--AGCCUGUGG-UGUGCGuu -5' |
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| 23440 | 3' | -54.2 | NC_005259.1 | + | 5015 | 0.66 | 0.82911 |
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Target: 5'- uGGUCGAgAUCGauccGGCcCCACACGaCAGc -3' miRNA: 3'- gUCAGCUgUAGC----CUGuGGUGUGC-GUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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