Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23440 | 3' | -54.2 | NC_005259.1 | + | 56804 | 0.72 | 0.462205 |
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Target: 5'- aCAGaUCGGgGUCGGugGCCACccauuGCGCGc -3' miRNA: 3'- -GUC-AGCUgUAGCCugUGGUG-----UGCGUu -5' |
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| 23440 | 3' | -54.2 | NC_005259.1 | + | 49296 | 0.72 | 0.442485 |
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Target: 5'- gUAGUCGACAucgUCGGGgucCAUCAgGCGCGAg -3' miRNA: 3'- -GUCAGCUGU---AGCCU---GUGGUgUGCGUU- -5' |
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| 23440 | 3' | -54.2 | NC_005259.1 | + | 45903 | 0.72 | 0.442485 |
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Target: 5'- uCGGUCGg---CGGGCACCGCugGUAGa -3' miRNA: 3'- -GUCAGCuguaGCCUGUGGUGugCGUU- -5' |
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| 23440 | 3' | -54.2 | NC_005259.1 | + | 42601 | 0.73 | 0.436662 |
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Target: 5'- gCAGUCGACAUCGG-CGgucaugagcccgaugUCGCGCGUAGg -3' miRNA: 3'- -GUCAGCUGUAGCCuGU---------------GGUGUGCGUU- -5' |
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| 23440 | 3' | -54.2 | NC_005259.1 | + | 16455 | 0.74 | 0.377435 |
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Target: 5'- -cGUCGGCAUCGGuACcCCGC-CGCAGa -3' miRNA: 3'- guCAGCUGUAGCC-UGuGGUGuGCGUU- -5' |
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| 23440 | 3' | -54.2 | NC_005259.1 | + | 11231 | 0.76 | 0.296304 |
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Target: 5'- gCAGUCcucgGGC-UCGGGCAUCGCGCGCAc -3' miRNA: 3'- -GUCAG----CUGuAGCCUGUGGUGUGCGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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