Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23440 | 5' | -55.8 | NC_005259.1 | + | 44657 | 0.66 | 0.782115 |
Target: 5'- gCCCGAcuCGAaCgccacacCGUUGGGCAGCGu -3' miRNA: 3'- -GGGCU--GCUaGaaga---GCAGCCCGUCGU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 19194 | 0.66 | 0.782115 |
Target: 5'- cCUCGACag-CgagCUCGUggugCGGGCGGCGa -3' miRNA: 3'- -GGGCUGcuaGaa-GAGCA----GCCCGUCGU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 67582 | 0.66 | 0.772407 |
Target: 5'- -aUGAUGAUCgacgCGUCGGGC-GCGa -3' miRNA: 3'- ggGCUGCUAGaagaGCAGCCCGuCGU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 30083 | 0.66 | 0.772407 |
Target: 5'- aCCCGAUG-UCgaccgccCUCGUCGGaGCuguguGCAu -3' miRNA: 3'- -GGGCUGCuAGaa-----GAGCAGCC-CGu----CGU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 64611 | 0.66 | 0.772407 |
Target: 5'- aCUGACGG-CgagCgCGaCGGGCAGCAg -3' miRNA: 3'- gGGCUGCUaGaa-GaGCaGCCCGUCGU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 7791 | 0.66 | 0.772407 |
Target: 5'- cCCCGGCGGggUUUUCUCGUuuguaaugacCGguaaugacccGGCGGCGg -3' miRNA: 3'- -GGGCUGCU--AGAAGAGCA----------GC----------CCGUCGU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 50687 | 0.66 | 0.771428 |
Target: 5'- gCCGACGAggcgCUgCUCGUCGucgcgcuugagccGGguGCu -3' miRNA: 3'- gGGCUGCUa---GAaGAGCAGC-------------CCguCGu -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 60283 | 0.66 | 0.752591 |
Target: 5'- gCCgGGCGGcCaUCgUCGUCGGuGCGGCc -3' miRNA: 3'- -GGgCUGCUaGaAG-AGCAGCC-CGUCGu -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 3305 | 0.66 | 0.752591 |
Target: 5'- cCUCGGCGAgcgCUgcgUCgacgugagCGcCGGGCAGCu -3' miRNA: 3'- -GGGCUGCUa--GA---AGa-------GCaGCCCGUCGu -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 39466 | 0.66 | 0.752591 |
Target: 5'- aCUGGCGAgccg-UCGUCGcGGUAGCAc -3' miRNA: 3'- gGGCUGCUagaagAGCAGC-CCGUCGU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 49716 | 0.67 | 0.732315 |
Target: 5'- aUCCGGCaGAUa--UUCGgCGGGCGGCGa -3' miRNA: 3'- -GGGCUG-CUAgaaGAGCaGCCCGUCGU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 62538 | 0.67 | 0.709576 |
Target: 5'- cCUCGACGGUCcgagagauagcCUCacCGGGCAGCGg -3' miRNA: 3'- -GGGCUGCUAGaa---------GAGcaGCCCGUCGU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 52744 | 0.67 | 0.707491 |
Target: 5'- gCCGGgauCGAUCUgcacaacaucgagCUCGUCGgugucGGCAGCGu -3' miRNA: 3'- gGGCU---GCUAGAa------------GAGCAGC-----CCGUCGU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 37583 | 0.67 | 0.701216 |
Target: 5'- gCCCGcgcCGAUCUgcaagCUgaaCGUCGGGCcgAGCu -3' miRNA: 3'- -GGGCu--GCUAGAa----GA---GCAGCCCG--UCGu -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 68691 | 0.67 | 0.701216 |
Target: 5'- uCUCGAUGAUCga---GgCGGGCAGCGc -3' miRNA: 3'- -GGGCUGCUAGaagagCaGCCCGUCGU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 7479 | 0.68 | 0.679092 |
Target: 5'- uCCCGACauaggcgGGUaaguuagUUCUUGUCGGGCGGgAg -3' miRNA: 3'- -GGGCUG-------CUAg------AAGAGCAGCCCGUCgU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 62708 | 0.68 | 0.669548 |
Target: 5'- aUCCGGCGAUCaggcgggggaUCUCGcUCGaccggaccGGCGGCAg -3' miRNA: 3'- -GGGCUGCUAGa---------AGAGC-AGC--------CCGUCGU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 41516 | 0.68 | 0.665298 |
Target: 5'- aCCGugGAUCUgcacggggaaaagCUCGUCGucGCAGUc -3' miRNA: 3'- gGGCugCUAGAa------------GAGCAGCc-CGUCGu -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 18705 | 0.68 | 0.637581 |
Target: 5'- -aCGGCGGUCUgacCUCGaucaUCGGGCcgcccGGCAu -3' miRNA: 3'- ggGCUGCUAGAa--GAGC----AGCCCG-----UCGU- -5' |
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23440 | 5' | -55.8 | NC_005259.1 | + | 49584 | 0.68 | 0.635446 |
Target: 5'- aCCGGCGggCggggucagcacCUCGaccUCGGGCAGCu -3' miRNA: 3'- gGGCUGCuaGaa---------GAGC---AGCCCGUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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