Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23443 | 3' | -50.7 | NC_005259.1 | + | 59866 | 0.66 | 0.947328 |
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Target: 5'- aUGCUg--GCUCguugugGCCGAAAccgGGGGCGa -3' miRNA: 3'- gACGAaugCGAG------UGGUUUU---UCCCGCa -5' |
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| 23443 | 3' | -50.7 | NC_005259.1 | + | 50082 | 0.66 | 0.947328 |
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Target: 5'- cCUGCggcaGCUCGCCGAu--GGuGCGc -3' miRNA: 3'- -GACGaaugCGAGUGGUUuuuCC-CGCa -5' |
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| 23443 | 3' | -50.7 | NC_005259.1 | + | 8612 | 0.66 | 0.942414 |
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Target: 5'- uUGCUUcAUGgUCACCGGAGAuccGGCGa -3' miRNA: 3'- gACGAA-UGCgAGUGGUUUUUc--CCGCa -5' |
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| 23443 | 3' | -50.7 | NC_005259.1 | + | 58773 | 0.66 | 0.931716 |
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Target: 5'- gCUGCggGCGCUgugCGCCGGucGAGGcGGUGa -3' miRNA: 3'- -GACGaaUGCGA---GUGGUU--UUUC-CCGCa -5' |
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| 23443 | 3' | -50.7 | NC_005259.1 | + | 18106 | 0.66 | 0.931716 |
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Target: 5'- -aGCcgucGCGCUCAUCGAcuacGAGGGCa- -3' miRNA: 3'- gaCGaa--UGCGAGUGGUUu---UUCCCGca -5' |
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| 23443 | 3' | -50.7 | NC_005259.1 | + | 2630 | 0.66 | 0.92593 |
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Target: 5'- -cGC--AC-CUCGCCGAAcAAGGGCGg -3' miRNA: 3'- gaCGaaUGcGAGUGGUUU-UUCCCGCa -5' |
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| 23443 | 3' | -50.7 | NC_005259.1 | + | 48623 | 0.66 | 0.92593 |
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Target: 5'- gUGCgcacUGC-CUCGCCGAGAcGGGCu- -3' miRNA: 3'- gACGa---AUGcGAGUGGUUUUuCCCGca -5' |
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| 23443 | 3' | -50.7 | NC_005259.1 | + | 24165 | 0.67 | 0.906819 |
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Target: 5'- aUGUcUACGCcaaccaCACCAAGGuGGGCGa -3' miRNA: 3'- gACGaAUGCGa-----GUGGUUUUuCCCGCa -5' |
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| 23443 | 3' | -50.7 | NC_005259.1 | + | 42673 | 0.67 | 0.899871 |
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Target: 5'- gCUGCUguugGCGauCUCGaccCCGuuGAGGGCGa -3' miRNA: 3'- -GACGAa---UGC--GAGU---GGUuuUUCCCGCa -5' |
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| 23443 | 3' | -50.7 | NC_005259.1 | + | 50521 | 0.67 | 0.892639 |
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Target: 5'- gCUGCaccucgacGCGCUCGCCuc---GGGCGa -3' miRNA: 3'- -GACGaa------UGCGAGUGGuuuuuCCCGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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