Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23446 | 5' | -65 | NC_005259.1 | + | 57645 | 0.66 | 0.289327 |
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Target: 5'- gUGCCCGccGGGUACagcagcacgucgaCCGuGUCCGGC-Ca -3' miRNA: 3'- gACGGGC--UCCGUGg------------GGC-CAGGCCGuG- -5' |
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| 23446 | 5' | -65 | NC_005259.1 | + | 42623 | 0.66 | 0.29135 |
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Target: 5'- -aGCCCGAugucgcgcguaGGCACCUCGGcCCcgaugugGGCGg -3' miRNA: 3'- gaCGGGCU-----------CCGUGGGGCCaGG-------CCGUg -5' |
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| 23446 | 5' | -65 | NC_005259.1 | + | 26642 | 0.66 | 0.292027 |
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Target: 5'- -gGCCUGGcGuGCGCUCggcaGGUUCGGCGCa -3' miRNA: 3'- gaCGGGCU-C-CGUGGGg---CCAGGCCGUG- -5' |
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| 23446 | 5' | -65 | NC_005259.1 | + | 41231 | 0.66 | 0.292027 |
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Target: 5'- cCUGCUCGGcggugacgauccGGUgcgccucaccGCCCCGGUCgagcacccucguCGGCGCa -3' miRNA: 3'- -GACGGGCU------------CCG----------UGGGGCCAG------------GCCGUG- -5' |
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| 23446 | 5' | -65 | NC_005259.1 | + | 60419 | 0.66 | 0.312902 |
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Target: 5'- uCUGCUCGGGGgugGCCCaCGGggCGGcCACg -3' miRNA: 3'- -GACGGGCUCCg--UGGG-GCCagGCC-GUG- -5' |
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| 23446 | 5' | -65 | NC_005259.1 | + | 51661 | 0.66 | 0.320108 |
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Target: 5'- uUGCCgCGcGGGCGCgUCGGgcaCgGGCGCg -3' miRNA: 3'- gACGG-GC-UCCGUGgGGCCa--GgCCGUG- -5' |
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| 23446 | 5' | -65 | NC_005259.1 | + | 67819 | 0.66 | 0.320835 |
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Target: 5'- -aGCCCGAucuuguccucgaccGGCAgCCCGGUgagcgggucgaccaaUCGGUGCc -3' miRNA: 3'- gaCGGGCU--------------CCGUgGGGCCA---------------GGCCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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