Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23446 | 5' | -65 | NC_005259.1 | + | 63656 | 0.7 | 0.179988 |
Target: 5'- gCUGgCCGGgacgccGGaCACCUCGGgcaCCGGCGCg -3' miRNA: 3'- -GACgGGCU------CC-GUGGGGCCa--GGCCGUG- -5' |
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23446 | 5' | -65 | NC_005259.1 | + | 4523 | 0.69 | 0.200814 |
Target: 5'- -cGCUgCGcGGCACCCCGGUcgauugugcccagugCgGGCACg -3' miRNA: 3'- gaCGG-GCuCCGUGGGGCCA---------------GgCCGUG- -5' |
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23446 | 5' | -65 | NC_005259.1 | + | 62406 | 0.68 | 0.213036 |
Target: 5'- cCUGCUCGAccacGGCACCCguaucggguggaGGUUCGGaCGCa -3' miRNA: 3'- -GACGGGCU----CCGUGGGg-----------CCAGGCC-GUG- -5' |
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23446 | 5' | -65 | NC_005259.1 | + | 46248 | 0.68 | 0.224796 |
Target: 5'- -cGCCUGAGGCacgucgACCCCgagGGUCUGcGCGa -3' miRNA: 3'- gaCGGGCUCCG------UGGGG---CCAGGC-CGUg -5' |
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23446 | 5' | -65 | NC_005259.1 | + | 57000 | 0.68 | 0.224796 |
Target: 5'- gCUGCCCGugcGaGCACCUcagcgCGGcUCGGCGCg -3' miRNA: 3'- -GACGGGCu--C-CGUGGG-----GCCaGGCCGUG- -5' |
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23446 | 5' | -65 | NC_005259.1 | + | 45379 | 0.68 | 0.230321 |
Target: 5'- uUGCCCGAGGaauuUCUCGGggucgccgCCGaGCGCg -3' miRNA: 3'- gACGGGCUCCgu--GGGGCCa-------GGC-CGUG- -5' |
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23446 | 5' | -65 | NC_005259.1 | + | 67819 | 0.66 | 0.320835 |
Target: 5'- -aGCCCGAucuuguccucgaccGGCAgCCCGGUgagcgggucgaccaaUCGGUGCc -3' miRNA: 3'- gaCGGGCU--------------CCGUgGGGCCA---------------GGCCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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