Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23448 | 5' | -60.1 | NC_005259.1 | + | 54683 | 0.66 | 0.476595 |
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Target: 5'- uCGGCGAGCgagaccgugucGAGGCgggugGCCUCaGccUGGUa -3' miRNA: 3'- -GCCGUUCGa----------CUCCGa----CGGAGcC--ACCA- -5' |
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| 23448 | 5' | -60.1 | NC_005259.1 | + | 8648 | 0.67 | 0.410785 |
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Target: 5'- gGGCAcgaGGCUGuggucGGGCUGCCacgCGGgcaacgGGg -3' miRNA: 3'- gCCGU---UCGAC-----UCCGACGGa--GCCa-----CCa -5' |
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| 23448 | 5' | -60.1 | NC_005259.1 | + | 33999 | 0.67 | 0.409886 |
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Target: 5'- gCGGUGAGCUGcaaccGGCUguauccgGCCUCGGcGGc -3' miRNA: 3'- -GCCGUUCGACu----CCGA-------CGGAGCCaCCa -5' |
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| 23448 | 5' | -60.1 | NC_005259.1 | + | 51772 | 0.69 | 0.350917 |
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Target: 5'- uCGGCGAGCgccuUGAGGUauucgGCgCUCGGcgGGUc -3' miRNA: 3'- -GCCGUUCG----ACUCCGa----CG-GAGCCa-CCA- -5' |
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| 23448 | 5' | -60.1 | NC_005259.1 | + | 27071 | 0.69 | 0.346083 |
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Target: 5'- aCGGCGAcaucgccgacauucuGCgGcGGCUGCCaugCGGUGGg -3' miRNA: 3'- -GCCGUU---------------CGaCuCCGACGGa--GCCACCa -5' |
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| 23448 | 5' | -60.1 | NC_005259.1 | + | 5528 | 0.71 | 0.244457 |
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Target: 5'- -cGCAAGCUGuGGCUGCuCUCGcccgaggccgcGUGGg -3' miRNA: 3'- gcCGUUCGACuCCGACG-GAGC-----------CACCa -5' |
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| 23448 | 5' | -60.1 | NC_005259.1 | + | 63859 | 0.72 | 0.215452 |
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Target: 5'- cCGGCGAGgUGGccGGCUgGCCUUGcGUGGg -3' miRNA: 3'- -GCCGUUCgACU--CCGA-CGGAGC-CACCa -5' |
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| 23448 | 5' | -60.1 | NC_005259.1 | + | 55029 | 0.79 | 0.073631 |
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Target: 5'- uCGGCAcgGGCUgccGAGGUgcuUGCCUCGGUGGc -3' miRNA: 3'- -GCCGU--UCGA---CUCCG---ACGGAGCCACCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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