Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23449 | 3' | -54.2 | NC_005259.1 | + | 7241 | 0.66 | 0.861177 |
Target: 5'- gGCAGccGCGCcgguGUCGAG----UGCGCCa -3' miRNA: 3'- -CGUCaaCGCG----CAGCUCcaauGCGCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 23293 | 0.66 | 0.818132 |
Target: 5'- gGUGGUUGgGUaUCGGGGUccgGCGCGgUCg -3' miRNA: 3'- -CGUCAACgCGcAGCUCCAa--UGCGC-GG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 41371 | 0.66 | 0.824468 |
Target: 5'- aCAGcgUGCG-GUCGAGcUUggccgguacccaugGCGCGCCg -3' miRNA: 3'- cGUCa-ACGCgCAGCUCcAA--------------UGCGCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 39089 | 0.66 | 0.844597 |
Target: 5'- aCAGUccguugaGCGgGUUGAGGUgcugcaauCGCGUCa -3' miRNA: 3'- cGUCAa------CGCgCAGCUCCAau------GCGCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 56084 | 0.66 | 0.844597 |
Target: 5'- aGCAGaucggUGCuCGUCGGGGccaccGCGUgGCCa -3' miRNA: 3'- -CGUCa----ACGcGCAGCUCCaa---UGCG-CGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 14077 | 0.66 | 0.844597 |
Target: 5'- gGCGGUggccuacgUGCGCagcgagGUCGAGGgUGCGgaaacgcaUGCCa -3' miRNA: 3'- -CGUCA--------ACGCG------CAGCUCCaAUGC--------GCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 52382 | 0.66 | 0.850501 |
Target: 5'- aGCGGcUGCGCaGccugccucgggccaUCGAGGUgUGgGCGCUc -3' miRNA: 3'- -CGUCaACGCG-C--------------AGCUCCA-AUgCGCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 49635 | 0.66 | 0.852169 |
Target: 5'- gGCGGcaGcCGCGUCGGcgauggcGGccucgaUACGCGCCu -3' miRNA: 3'- -CGUCaaC-GCGCAGCU-------CCa-----AUGCGCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 8742 | 0.66 | 0.852999 |
Target: 5'- gGCAGUcaGCcCGUCGAGGUcgAgGCccuugGCCa -3' miRNA: 3'- -CGUCAa-CGcGCAGCUCCAa-UgCG-----CGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 27145 | 0.66 | 0.852999 |
Target: 5'- gGCGGcUGCgGUGUUGGGGccaACGaUGCCg -3' miRNA: 3'- -CGUCaACG-CGCAGCUCCaa-UGC-GCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 56918 | 0.66 | 0.860369 |
Target: 5'- aGCAGgcgUGCccGCGUCGGugagcaugcGGUUGgcgaacuCGCGCUc -3' miRNA: 3'- -CGUCa--ACG--CGCAGCU---------CCAAU-------GCGCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 28899 | 0.66 | 0.861177 |
Target: 5'- aGCAGaUUGUcgGCGaCGAGGa-ACGcCGCCu -3' miRNA: 3'- -CGUC-AACG--CGCaGCUCCaaUGC-GCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 31065 | 0.66 | 0.861177 |
Target: 5'- aUAGUUGauCGggucggCGAGGUUcccgaGCGCGUCg -3' miRNA: 3'- cGUCAACgcGCa-----GCUCCAA-----UGCGCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 48451 | 0.66 | 0.861177 |
Target: 5'- aGCGGcaGCGCGUUccacc-GCGCGCCg -3' miRNA: 3'- -CGUCaaCGCGCAGcuccaaUGCGCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 66028 | 0.67 | 0.807056 |
Target: 5'- cGCAcguGUUGCGCG-CGAGcucgacguagACGcCGCCg -3' miRNA: 3'- -CGU---CAACGCGCaGCUCcaa-------UGC-GCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 53194 | 0.67 | 0.803306 |
Target: 5'- cGCGGUguccccgGCgaucagauucGCGUCgcccgcgcccugcuuGAGGUU-CGCGCCg -3' miRNA: 3'- -CGUCAa------CG----------CGCAG---------------CUCCAAuGCGCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 35243 | 0.67 | 0.780249 |
Target: 5'- uGCAGUcUGCGCacucgCGuuGUU-CGCGCCc -3' miRNA: 3'- -CGUCA-ACGCGca---GCucCAAuGCGCGG- -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 41595 | 0.67 | 0.770381 |
Target: 5'- aGCGGUcGCGCGUCGGuGUUucggauCGCGa- -3' miRNA: 3'- -CGUCAaCGCGCAGCUcCAAu-----GCGCgg -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 60759 | 0.67 | 0.770381 |
Target: 5'- uGCAGUcgGCuCGgCGAGGUUGggcUGCGCg -3' miRNA: 3'- -CGUCAa-CGcGCaGCUCCAAU---GCGCGg -5' |
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23449 | 3' | -54.2 | NC_005259.1 | + | 53023 | 0.68 | 0.760375 |
Target: 5'- uGCGGUg--GCGUCGAGGcauCGUGaCCu -3' miRNA: 3'- -CGUCAacgCGCAGCUCCaauGCGC-GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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