Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23452 | 3' | -62.5 | NC_005259.1 | + | 23354 | 0.66 | 0.38674 |
Target: 5'- -aGGCUGCCuGCGaGGCCgccgAGGCAu -3' miRNA: 3'- cgCCGACGG-CGCaCCGGgaugUCCGU- -5' |
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23452 | 3' | -62.5 | NC_005259.1 | + | 54166 | 0.66 | 0.392716 |
Target: 5'- -gGGCauaCCGCaacuguugaucuugGUGGCCCU-CAGGCGg -3' miRNA: 3'- cgCCGac-GGCG--------------CACCGGGAuGUCCGU- -5' |
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23452 | 3' | -62.5 | NC_005259.1 | + | 4528 | 0.66 | 0.403974 |
Target: 5'- cGCGGCaccccgGUCGauUGuGCCCagUGCGGGCAc -3' miRNA: 3'- -CGCCGa-----CGGCgcAC-CGGG--AUGUCCGU- -5' |
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23452 | 3' | -62.5 | NC_005259.1 | + | 47763 | 0.66 | 0.403974 |
Target: 5'- aGCGGCUG-CGCGaUGGCCUUuuggaaaucgcGCAG-CGg -3' miRNA: 3'- -CGCCGACgGCGC-ACCGGGA-----------UGUCcGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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