Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23452 | 5' | -54.9 | NC_005259.1 | + | 27910 | 0.66 | 0.802702 |
Target: 5'- gCCgagACCgcGCGGGUGGU-AGCGGuucgGCg -3' miRNA: 3'- -GGa--UGGa-UGUCCACCGgUCGCCua--UG- -5' |
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23452 | 5' | -54.9 | NC_005259.1 | + | 56039 | 0.66 | 0.802702 |
Target: 5'- aCUuCCU-CGGGcGGCU-GCGGGUACu -3' miRNA: 3'- gGAuGGAuGUCCaCCGGuCGCCUAUG- -5' |
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23452 | 5' | -54.9 | NC_005259.1 | + | 26438 | 0.66 | 0.78339 |
Target: 5'- aCC-GCCgaGCGGGcgGGCCGGUGGGc-- -3' miRNA: 3'- -GGaUGGa-UGUCCa-CCGGUCGCCUaug -5' |
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23452 | 5' | -54.9 | NC_005259.1 | + | 37757 | 0.66 | 0.78339 |
Target: 5'- --gACCaGCGcGGUGGCCccuGCGGGcACg -3' miRNA: 3'- ggaUGGaUGU-CCACCGGu--CGCCUaUG- -5' |
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23452 | 5' | -54.9 | NC_005259.1 | + | 38961 | 0.66 | 0.763463 |
Target: 5'- gCCgagaGCCUGCAcGaUGGCaucGCGGAUACc -3' miRNA: 3'- -GGa---UGGAUGUcC-ACCGgu-CGCCUAUG- -5' |
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23452 | 5' | -54.9 | NC_005259.1 | + | 60418 | 0.68 | 0.690138 |
Target: 5'- aUCUGCUcGgGGGUGGCCcacggGGCGGccACg -3' miRNA: 3'- -GGAUGGaUgUCCACCGG-----UCGCCuaUG- -5' |
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23452 | 5' | -54.9 | NC_005259.1 | + | 8971 | 0.68 | 0.646788 |
Target: 5'- aCCgUGCCgggACguAGGU-GCCAGCGGcgACg -3' miRNA: 3'- -GG-AUGGa--UG--UCCAcCGGUCGCCuaUG- -5' |
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23452 | 5' | -54.9 | NC_005259.1 | + | 9977 | 0.69 | 0.624994 |
Target: 5'- aCCgcACCgguCgAGGUGGCCAGcCGGggACu -3' miRNA: 3'- -GGa-UGGau-G-UCCACCGGUC-GCCuaUG- -5' |
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23452 | 5' | -54.9 | NC_005259.1 | + | 13198 | 0.69 | 0.603223 |
Target: 5'- cUCUACCaagcACAGcGgGGCCAGCGGuggGCc -3' miRNA: 3'- -GGAUGGa---UGUC-CaCCGGUCGCCua-UG- -5' |
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23452 | 5' | -54.9 | NC_005259.1 | + | 29086 | 0.7 | 0.538759 |
Target: 5'- gCUGCCaaggcCGGGUGGUgAGCuGAUGCg -3' miRNA: 3'- gGAUGGau---GUCCACCGgUCGcCUAUG- -5' |
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23452 | 5' | -54.9 | NC_005259.1 | + | 36004 | 0.7 | 0.52822 |
Target: 5'- gCCggaCUGCaccgAGGUGGCCAGCGaGGUcgACa -3' miRNA: 3'- -GGaugGAUG----UCCACCGGUCGC-CUA--UG- -5' |
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23452 | 5' | -54.9 | NC_005259.1 | + | 63854 | 0.71 | 0.507382 |
Target: 5'- gCgUACCgGCgAGGUGGCCGGCuGGccuUGCg -3' miRNA: 3'- -GgAUGGaUG-UCCACCGGUCG-CCu--AUG- -5' |
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23452 | 5' | -54.9 | NC_005259.1 | + | 58274 | 0.74 | 0.356432 |
Target: 5'- -aUGCCcgacagauCAGGUucgcgaGGCCAGCGGAUACc -3' miRNA: 3'- ggAUGGau------GUCCA------CCGGUCGCCUAUG- -5' |
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23452 | 5' | -54.9 | NC_005259.1 | + | 35648 | 0.78 | 0.199851 |
Target: 5'- gCUGCCgcugGCgaGGGUGGCCAGCGcGGUGg -3' miRNA: 3'- gGAUGGa---UG--UCCACCGGUCGC-CUAUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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