Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23455 | 3' | -53.3 | NC_005259.1 | + | 30616 | 0.66 | 0.866611 |
Target: 5'- cUCGCCGUUucuGGUGAUCGA--GUGAa -3' miRNA: 3'- uAGUGGCGGuu-CCACUAGUUgcCACU- -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 25925 | 0.66 | 0.866611 |
Target: 5'- -cCGCCGCCGGuccGGUGAgcgCAcgcCGGUGc -3' miRNA: 3'- uaGUGGCGGUU---CCACUa--GUu--GCCACu -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 4471 | 0.66 | 0.858394 |
Target: 5'- -----gGCCAAGGccAUCGGCGGUGGa -3' miRNA: 3'- uaguggCGGUUCCacUAGUUGCCACU- -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 6172 | 0.66 | 0.849937 |
Target: 5'- --aACgGUCGAGGUGAccaAugGGUGAu -3' miRNA: 3'- uagUGgCGGUUCCACUag-UugCCACU- -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 36526 | 0.66 | 0.849079 |
Target: 5'- -cCAcCCGCgcggugugacacgCGGGGUGAUCuGCGGUGu -3' miRNA: 3'- uaGU-GGCG-------------GUUCCACUAGuUGCCACu -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 43886 | 0.66 | 0.849079 |
Target: 5'- cGUUGCCGCCGAcauugcccgcgccGGcUGAUCGAUGGg-- -3' miRNA: 3'- -UAGUGGCGGUU-------------CC-ACUAGUUGCCacu -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 47167 | 0.66 | 0.832338 |
Target: 5'- gAUCACCGUagcGGcgaaGGUCGGCGGUGc -3' miRNA: 3'- -UAGUGGCGguuCCa---CUAGUUGCCACu -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 47467 | 0.66 | 0.823213 |
Target: 5'- gGUCAgCCaCCGAGGUGAUCGcgucaccgaggcGCGGcagGAa -3' miRNA: 3'- -UAGU-GGcGGUUCCACUAGU------------UGCCa--CU- -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 19154 | 0.66 | 0.822289 |
Target: 5'- -aCACCGaUCAGGGUcGAgguucgucggcgcUCAACGGUGc -3' miRNA: 3'- uaGUGGC-GGUUCCA-CU-------------AGUUGCCACu -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 25190 | 0.66 | 0.822289 |
Target: 5'- -cCGCCGCCGAucccaccGG-GGUCAGCGGc-- -3' miRNA: 3'- uaGUGGCGGUU-------CCaCUAGUUGCCacu -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 45177 | 0.67 | 0.813885 |
Target: 5'- uGUUGCgCGCCGAGG---UCAgcGCGGUGAu -3' miRNA: 3'- -UAGUG-GCGGUUCCacuAGU--UGCCACU- -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 2007 | 0.67 | 0.813885 |
Target: 5'- -aCGCgGCCAucGGGUGGcgcaccggcaccUCAguGCGGUGGg -3' miRNA: 3'- uaGUGgCGGU--UCCACU------------AGU--UGCCACU- -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 36440 | 0.67 | 0.804363 |
Target: 5'- -gCGUCGCCGAGGUcGAccgcgUCGAgGGUGAg -3' miRNA: 3'- uaGUGGCGGUUCCA-CU-----AGUUgCCACU- -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 37476 | 0.67 | 0.804363 |
Target: 5'- -aCGCCGCCGuuGGcUGAcccaccccCGGCGGUGAg -3' miRNA: 3'- uaGUGGCGGUu-CC-ACUa-------GUUGCCACU- -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 3804 | 0.67 | 0.804363 |
Target: 5'- gAUUGCCGCCAccaAGGUGAcCAcccACGGg-- -3' miRNA: 3'- -UAGUGGCGGU---UCCACUaGU---UGCCacu -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 8698 | 0.67 | 0.774745 |
Target: 5'- uGUCGCUGUCGAGGUcGUC-GCGGUc- -3' miRNA: 3'- -UAGUGGCGGUUCCAcUAGuUGCCAcu -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 55037 | 0.67 | 0.774745 |
Target: 5'- --gGCUGCCGAGGUGcuugCcuCGGUGGc -3' miRNA: 3'- uagUGGCGGUUCCACua--GuuGCCACU- -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 20972 | 0.68 | 0.764558 |
Target: 5'- -gUAUCGCCGGGGUGA-CAacuGCGuGUGGg -3' miRNA: 3'- uaGUGGCGGUUCCACUaGU---UGC-CACU- -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 41481 | 0.68 | 0.764558 |
Target: 5'- -aCGCCGUCGAGcacGAccUCGGCGGUGGc -3' miRNA: 3'- uaGUGGCGGUUCca-CU--AGUUGCCACU- -5' |
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23455 | 3' | -53.3 | NC_005259.1 | + | 30004 | 0.68 | 0.743782 |
Target: 5'- gGUCACCacgaCAAGGUGAcCAccgccgggGCGGUGGg -3' miRNA: 3'- -UAGUGGcg--GUUCCACUaGU--------UGCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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