Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23456 | 5' | -50.7 | NC_005259.1 | + | 27996 | 0.66 | 0.957795 |
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Target: 5'- uGGU-GCGgguGCCGggGCGGGGUCGCCGGg- -3' miRNA: 3'- -CCAgUGC---UGGC--UGUUCUAGCGGUUac -5' |
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| 23456 | 5' | -50.7 | NC_005259.1 | + | 17341 | 0.66 | 0.957795 |
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Target: 5'- cGGUCaACGucGCCGuCGAGGUCGgCGGc- -3' miRNA: 3'- -CCAG-UGC--UGGCuGUUCUAGCgGUUac -5' |
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| 23456 | 5' | -50.7 | NC_005259.1 | + | 2733 | 0.66 | 0.961689 |
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Target: 5'- --aCACcACCGAugcCGAGGUCGCCGucGUGc -3' miRNA: 3'- ccaGUGcUGGCU---GUUCUAGCGGU--UAC- -5' |
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| 23456 | 5' | -50.7 | NC_005259.1 | + | 3073 | 0.66 | 0.961689 |
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Target: 5'- gGGcCGaGGCCGACAAGAgagccgCGCagCGAUGa -3' miRNA: 3'- -CCaGUgCUGGCUGUUCUa-----GCG--GUUAC- -5' |
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| 23456 | 5' | -50.7 | NC_005259.1 | + | 21036 | 0.66 | 0.965327 |
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Target: 5'- ---gAUGACCGGCGcgcguucaAGGUCGCCGc-- -3' miRNA: 3'- ccagUGCUGGCUGU--------UCUAGCGGUuac -5' |
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| 23456 | 5' | -50.7 | NC_005259.1 | + | 19688 | 0.66 | 0.965327 |
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Target: 5'- aGGUCGgGAUCGAgCAGGcUCGCaCGuUGg -3' miRNA: 3'- -CCAGUgCUGGCU-GUUCuAGCG-GUuAC- -5' |
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| 23456 | 5' | -50.7 | NC_005259.1 | + | 13830 | 0.66 | 0.965327 |
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Target: 5'- cGUgAUGACCGACGGGcacAUUGCCc--- -3' miRNA: 3'- cCAgUGCUGGCUGUUC---UAGCGGuuac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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