Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23456 | 5' | -50.7 | NC_005259.1 | + | 8061 | 0.72 | 0.722548 |
|
Target: 5'- cGGUCGCcGCCGACAucgAGAUCGUg---- -3' miRNA: 3'- -CCAGUGcUGGCUGU---UCUAGCGguuac -5' |
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| 23456 | 5' | -50.7 | NC_005259.1 | + | 6448 | 0.74 | 0.601673 |
|
Target: 5'- cGUCGCgcuGAUCGGCAGcGUCGCCGGUGc -3' miRNA: 3'- cCAGUG---CUGGCUGUUcUAGCGGUUAC- -5' |
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| 23456 | 5' | -50.7 | NC_005259.1 | + | 3165 | 0.69 | 0.858394 |
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Target: 5'- cGGUgAUGcCCGGCGaugucaAGGUCGUCGGUGc -3' miRNA: 3'- -CCAgUGCuGGCUGU------UCUAGCGGUUAC- -5' |
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| 23456 | 5' | -50.7 | NC_005259.1 | + | 3073 | 0.66 | 0.961689 |
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Target: 5'- gGGcCGaGGCCGACAAGAgagccgCGCagCGAUGa -3' miRNA: 3'- -CCaGUgCUGGCUGUUCUa-----GCG--GUUAC- -5' |
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| 23456 | 5' | -50.7 | NC_005259.1 | + | 2733 | 0.66 | 0.961689 |
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Target: 5'- --aCACcACCGAugcCGAGGUCGCCGucGUGc -3' miRNA: 3'- ccaGUGcUGGCU---GUUCUAGCGGU--UAC- -5' |
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| 23456 | 5' | -50.7 | NC_005259.1 | + | 2591 | 0.68 | 0.90386 |
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Target: 5'- cGUCGCGACgagGACAcGAUCuCCGGUGu -3' miRNA: 3'- cCAGUGCUGg--CUGUuCUAGcGGUUAC- -5' |
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| 23456 | 5' | -50.7 | NC_005259.1 | + | 1805 | 0.71 | 0.774744 |
|
Target: 5'- uGUCAUGGCCGACGcGAgcaauccCGCCGAc- -3' miRNA: 3'- cCAGUGCUGGCUGUuCUa------GCGGUUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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