Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23457 | 5' | -53.8 | NC_005259.1 | + | 67648 | 0.79 | 0.182471 |
Target: 5'- uUGCGGUACAGCGCGCUGuCGGCu-- -3' miRNA: 3'- cGCGUCAUGUUGCGCGACcGCUGuuu -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 21392 | 0.76 | 0.293031 |
Target: 5'- -gGCGGUGCAACGCGCaUGG-GACGc- -3' miRNA: 3'- cgCGUCAUGUUGCGCG-ACCgCUGUuu -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 44156 | 0.75 | 0.323784 |
Target: 5'- cGUGCGGUcgaGCAGCGCGCcGGUGuCGGAg -3' miRNA: 3'- -CGCGUCA---UGUUGCGCGaCCGCuGUUU- -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 39826 | 0.75 | 0.331839 |
Target: 5'- aGCGCA-UugAGCGCGUUGGuCGGCGAc -3' miRNA: 3'- -CGCGUcAugUUGCGCGACC-GCUGUUu -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 65781 | 0.73 | 0.439609 |
Target: 5'- uCGCAGUGCGccauggccucgACGCGCUGGuCGAa--- -3' miRNA: 3'- cGCGUCAUGU-----------UGCGCGACC-GCUguuu -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 59286 | 0.72 | 0.490032 |
Target: 5'- aGCGguGUGCAgaccuACGCuGUcgUGGCGGCAGc -3' miRNA: 3'- -CGCguCAUGU-----UGCG-CG--ACCGCUGUUu -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 66254 | 0.71 | 0.553818 |
Target: 5'- aCGCAGcGCGGCccGCGCcgGGCGGCGGc -3' miRNA: 3'- cGCGUCaUGUUG--CGCGa-CCGCUGUUu -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 10315 | 0.69 | 0.616512 |
Target: 5'- uCGUAGUcgucaucgggcucgGCGACGgGCUcGGCGGCAGc -3' miRNA: 3'- cGCGUCA--------------UGUUGCgCGA-CCGCUGUUu -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 4509 | 0.69 | 0.619839 |
Target: 5'- cGCGcCGGga-GugGCGCUGcGCGGCAc- -3' miRNA: 3'- -CGC-GUCaugUugCGCGAC-CGCUGUuu -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 50511 | 0.69 | 0.640923 |
Target: 5'- uGgGCGGUGCGcugcaccucgACGCGCUcgccucgGGCGAgGAAa -3' miRNA: 3'- -CgCGUCAUGU----------UGCGCGA-------CCGCUgUUU- -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 62847 | 0.68 | 0.686222 |
Target: 5'- cGCGCGGUugacgaGCAGC-CGCUcGGCG-CGAGg -3' miRNA: 3'- -CGCGUCA------UGUUGcGCGA-CCGCuGUUU- -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 13465 | 0.67 | 0.740183 |
Target: 5'- gGCGCuGGUGCucacCGCGCUGccCGACGAu -3' miRNA: 3'- -CGCG-UCAUGuu--GCGCGACc-GCUGUUu -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 17593 | 0.67 | 0.750689 |
Target: 5'- cGCGCAaccuCGGCGCGaugacguggGGCGGCGAGg -3' miRNA: 3'- -CGCGUcau-GUUGCGCga-------CCGCUGUUU- -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 67950 | 0.67 | 0.761074 |
Target: 5'- uUGCGGUGCGgguugugcuguaGCGCGCgcagcGGCGugGc- -3' miRNA: 3'- cGCGUCAUGU------------UGCGCGa----CCGCugUuu -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 61659 | 0.67 | 0.780423 |
Target: 5'- -aGCu---CAACGCGCUgcccaucGGCGGCAAAa -3' miRNA: 3'- cgCGucauGUUGCGCGA-------CCGCUGUUU- -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 28312 | 0.67 | 0.781427 |
Target: 5'- uGCGCAGcguagauCggUGUGCcguccuUGGCGGCGAAa -3' miRNA: 3'- -CGCGUCau-----GuuGCGCG------ACCGCUGUUU- -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 62013 | 0.67 | 0.781427 |
Target: 5'- uGCGguGUACccgcuCGCGCUGuGUGuCGAc -3' miRNA: 3'- -CGCguCAUGuu---GCGCGAC-CGCuGUUu -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 27739 | 0.66 | 0.798236 |
Target: 5'- cGCGCccgcugcugcccacGGUGCAuCGCcCUcGGCGACGAc -3' miRNA: 3'- -CGCG--------------UCAUGUuGCGcGA-CCGCUGUUu -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 39297 | 0.66 | 0.798236 |
Target: 5'- cGCGUcaucgaAGUGCAcgacgcccgcugucGCGcCGCUGGUGACc-- -3' miRNA: 3'- -CGCG------UCAUGU--------------UGC-GCGACCGCUGuuu -5' |
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23457 | 5' | -53.8 | NC_005259.1 | + | 17186 | 0.66 | 0.810748 |
Target: 5'- gGCGCAGgaaGCcGCGCGUgaGGCGGguAGa -3' miRNA: 3'- -CGCGUCa--UGuUGCGCGa-CCGCUguUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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