miRNA: 3'- -CGCGUCA---UGUUGCGCGaCCGCuGUUU- -5'
miRNA: 3'- cgCGUCAUGUUGCGCG-ACCgCUGUuu -5'
Results 21 - 22 of 22 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23457 | 5' | -53.8 | NC_005259.1 | + | 44156 | 0.75 | 0.323784 |
|
Target: 5'- cGUGCGGUcgaGCAGCGCGCcGGUGuCGGAg -3' miRNA: 3'- -CGCGUCA---UGUUGCGCGaCCGCuGUUU- -5' |
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| 23457 | 5' | -53.8 | NC_005259.1 | + | 21392 | 0.76 | 0.293031 |
|
Target: 5'- -gGCGGUGCAACGCGCaUGG-GACGc- -3' miRNA: 3'- cgCGUCAUGUUGCGCG-ACCgCUGUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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