Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23458 | 3' | -56.7 | NC_005259.1 | + | 50494 | 0.68 | 0.599815 |
Target: 5'- -cGAUGaGcCGGuCGAUguGG-GCGGUGCg -3' miRNA: 3'- gaCUAC-C-GCU-GCUAguCCgCGCCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 32897 | 0.65 | 0.734218 |
Target: 5'- -cGggGGCGAgggUGAgccacccggcccgcUCGGGCGCGGcgGUa -3' miRNA: 3'- gaCuaCCGCU---GCU--------------AGUCCGCGCCa-CG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 27386 | 0.66 | 0.727024 |
Target: 5'- gUGAccGGCGGCGGUgucGGCaGCGGcgGCa -3' miRNA: 3'- gACUa-CCGCUGCUAgu-CCG-CGCCa-CG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 979 | 0.66 | 0.727024 |
Target: 5'- ----cGGCGACGAgacaccggCAGccGUGUGGUGUa -3' miRNA: 3'- gacuaCCGCUGCUa-------GUC--CGCGCCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 56502 | 0.66 | 0.716673 |
Target: 5'- -cGGUGGCGAUGAuucccUCAGGCauUGGUa- -3' miRNA: 3'- gaCUACCGCUGCU-----AGUCCGc-GCCAcg -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 24026 | 0.66 | 0.706241 |
Target: 5'- -gGGcUGGCGAaauggaaccgcCGcaacGUCGucGGCGCGGUGCu -3' miRNA: 3'- gaCU-ACCGCU-----------GC----UAGU--CCGCGCCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 50143 | 0.66 | 0.706241 |
Target: 5'- -cGAuUGGUuGGCGAUCcGGCGCGuGaGCg -3' miRNA: 3'- gaCU-ACCG-CUGCUAGuCCGCGC-CaCG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 61030 | 0.66 | 0.705194 |
Target: 5'- -cGGUGGCGGCGAggucgaccgaCGGGCGgcucaccuugugcCGG-GCa -3' miRNA: 3'- gaCUACCGCUGCUa---------GUCCGC-------------GCCaCG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 46968 | 0.66 | 0.695742 |
Target: 5'- uUGuUGGCcacgccgccGAUGAUCGGGCcGagGGUGCg -3' miRNA: 3'- gACuACCG---------CUGCUAGUCCG-Cg-CCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 20511 | 0.66 | 0.695742 |
Target: 5'- ----cGGCGACGGUgucccgagcaCGGGUGCGcUGCc -3' miRNA: 3'- gacuaCCGCUGCUA----------GUCCGCGCcACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 58919 | 0.66 | 0.685185 |
Target: 5'- ----cGGCGGucuugucguCGG-CGGGCGCGGUGg -3' miRNA: 3'- gacuaCCGCU---------GCUaGUCCGCGCCACg -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 23127 | 0.66 | 0.685185 |
Target: 5'- gCUGuccGGCaGCGAUguGaCGUGGUGCg -3' miRNA: 3'- -GACua-CCGcUGCUAguCcGCGCCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 55105 | 0.67 | 0.653264 |
Target: 5'- -cGAUGGCGGCGAaaaaugCAccGGCuGCcGUGCc -3' miRNA: 3'- gaCUACCGCUGCUa-----GU--CCG-CGcCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 62699 | 0.67 | 0.642574 |
Target: 5'- -cGGUGucccaucCGGCGAUCAGGCGgGGgaucucGCu -3' miRNA: 3'- gaCUACc------GCUGCUAGUCCGCgCCa-----CG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 3167 | 0.67 | 0.621176 |
Target: 5'- gUGAUgcccGGCGAUGucaAGGuCGuCGGUGCg -3' miRNA: 3'- gACUA----CCGCUGCuagUCC-GC-GCCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 23261 | 0.67 | 0.621176 |
Target: 5'- -cGGUGGCcACcaugagcaccaaGAcCGGGCGCGGUGg -3' miRNA: 3'- gaCUACCGcUG------------CUaGUCCGCGCCACg -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 54021 | 0.81 | 0.098208 |
Target: 5'- -----aGCGACGcUCGGGCGCGGUGCg -3' miRNA: 3'- gacuacCGCUGCuAGUCCGCGCCACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 60344 | 0.68 | 0.589169 |
Target: 5'- ----aGGCGGCGAUCAGGuCGaGGcccUGCc -3' miRNA: 3'- gacuaCCGCUGCUAGUCC-GCgCC---ACG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 54508 | 0.68 | 0.589169 |
Target: 5'- --cAUGGCGGCGAUguGGgGUGGcggGUc -3' miRNA: 3'- gacUACCGCUGCUAguCCgCGCCa--CG- -5' |
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23458 | 3' | -56.7 | NC_005259.1 | + | 67582 | 0.68 | 0.578557 |
Target: 5'- aUGAUGauCGACGcGUCGGGCGCGagacGUGUu -3' miRNA: 3'- gACUACc-GCUGC-UAGUCCGCGC----CACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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