Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23458 | 5' | -51.6 | NC_005259.1 | + | 16688 | 0.66 | 0.923773 |
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Target: 5'- -----cGGCGAUC---CugGGCACCGa -3' miRNA: 3'- gauguuCCGCUAGauaGugCCGUGGU- -5' |
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| 23458 | 5' | -51.6 | NC_005259.1 | + | 42768 | 0.67 | 0.890429 |
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Target: 5'- uCUGCucGGCGGUC--UCGgGGcCGCCAc -3' miRNA: 3'- -GAUGuuCCGCUAGauAGUgCC-GUGGU- -5' |
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| 23458 | 5' | -51.6 | NC_005259.1 | + | 38280 | 0.68 | 0.841599 |
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Target: 5'- --cCGAGGUGAgaaUGUCAuCGGCGCUg -3' miRNA: 3'- gauGUUCCGCUag-AUAGU-GCCGUGGu -5' |
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| 23458 | 5' | -51.6 | NC_005259.1 | + | 56570 | 0.68 | 0.832618 |
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Target: 5'- -----cGGUGAUC-GUCGCGGCugCGa -3' miRNA: 3'- gauguuCCGCUAGaUAGUGCCGugGU- -5' |
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| 23458 | 5' | -51.6 | NC_005259.1 | + | 51100 | 0.69 | 0.804407 |
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Target: 5'- -aACGAGGgcggGAUCUGUC-CGGCagGCCAu -3' miRNA: 3'- gaUGUUCCg---CUAGAUAGuGCCG--UGGU- -5' |
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| 23458 | 5' | -51.6 | NC_005259.1 | + | 2039 | 0.69 | 0.804407 |
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Target: 5'- gUGCGguGGGCGGUCa--C-CGGCACCGa -3' miRNA: 3'- gAUGU--UCCGCUAGauaGuGCCGUGGU- -5' |
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| 23458 | 5' | -51.6 | NC_005259.1 | + | 58556 | 0.7 | 0.721842 |
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Target: 5'- uUACGgcucGGGCGGUUgggccggAUCGCGGCGCg- -3' miRNA: 3'- gAUGU----UCCGCUAGa------UAGUGCCGUGgu -5' |
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| 23458 | 5' | -51.6 | NC_005259.1 | + | 5008 | 0.71 | 0.700042 |
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Target: 5'- -gGCAAGGUGGUCgagAUCGaucCGGCcCCAc -3' miRNA: 3'- gaUGUUCCGCUAGa--UAGU---GCCGuGGU- -5' |
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| 23458 | 5' | -51.6 | NC_005259.1 | + | 22747 | 0.72 | 0.606562 |
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Target: 5'- aUACGAGGCGuaccgaaACGGCACCGc -3' miRNA: 3'- gAUGUUCCGCuagauagUGCCGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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