Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23459 | 5' | -56.6 | NC_005259.1 | + | 58952 | 0.75 | 0.23812 |
Target: 5'- cGUCGGCcuuGGCcagcagGUCGGGCaGCUCGUCGGc -3' miRNA: 3'- -UAGCCG---CUG------UAGCCCGcUGAGUAGCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 10757 | 0.75 | 0.269735 |
Target: 5'- --gGGCGAUcaucauGUCGGGCGGgUCAUCGa -3' miRNA: 3'- uagCCGCUG------UAGCCCGCUgAGUAGCc -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 10344 | 0.74 | 0.283299 |
Target: 5'- cUCGGCGGCAgCGGGCucgaCAUCGGg -3' miRNA: 3'- uAGCCGCUGUaGCCCGcugaGUAGCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 68317 | 0.74 | 0.312032 |
Target: 5'- -cCGGCGACGggaucucgaugaUgGGGCucagcGGCUCAUCGGg -3' miRNA: 3'- uaGCCGCUGU------------AgCCCG-----CUGAGUAGCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 10932 | 0.73 | 0.350983 |
Target: 5'- cUCGGCGAacUCGGuGuCGAcCUCGUCGGu -3' miRNA: 3'- uAGCCGCUguAGCC-C-GCU-GAGUAGCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 61028 | 0.72 | 0.368342 |
Target: 5'- cUCGGUGGCGgcgaggucgaccgaCGGGCGGCUCAccuugugccgggcaUCGGc -3' miRNA: 3'- uAGCCGCUGUa-------------GCCCGCUGAGU--------------AGCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 27071 | 0.72 | 0.37596 |
Target: 5'- -aCGGCGACAUCGccgacauucugcGGCGGCUgcCAUgCGGu -3' miRNA: 3'- uaGCCGCUGUAGC------------CCGCUGA--GUA-GCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 11779 | 0.72 | 0.384548 |
Target: 5'- -aCGGCGAgggagucccacaCAUCGGGCGcgacccgcuugaGCUCGUCGc -3' miRNA: 3'- uaGCCGCU------------GUAGCCCGC------------UGAGUAGCc -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 54512 | 0.71 | 0.448161 |
Target: 5'- -gCGGCGAUGUgGGGUGGCggGUCGa -3' miRNA: 3'- uaGCCGCUGUAgCCCGCUGagUAGCc -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 64149 | 0.7 | 0.466413 |
Target: 5'- cGUCGGCGAUgcccuugacguaGUC-GGCGACUgucugugCGUCGGg -3' miRNA: 3'- -UAGCCGCUG------------UAGcCCGCUGA-------GUAGCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 44263 | 0.7 | 0.467384 |
Target: 5'- aGUCGGCGACAUCGaGGcCGAacgCcUUGGc -3' miRNA: 3'- -UAGCCGCUGUAGC-CC-GCUga-GuAGCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 40110 | 0.69 | 0.521265 |
Target: 5'- aGUCGGCGAaugcgcucguggaauUggCGGGCGAgCUCgccGUCGGu -3' miRNA: 3'- -UAGCCGCU---------------GuaGCCCGCU-GAG---UAGCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 34028 | 0.69 | 0.527408 |
Target: 5'- cUCGGCGGCgugcGUCGGcGCGuuCUUGUUGGc -3' miRNA: 3'- uAGCCGCUG----UAGCC-CGCu-GAGUAGCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 18705 | 0.69 | 0.558494 |
Target: 5'- -aCGGCGGucugaccucgauCAUCGGGCcGCccggCAUCGGu -3' miRNA: 3'- uaGCCGCU------------GUAGCCCGcUGa---GUAGCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 60589 | 0.69 | 0.568976 |
Target: 5'- -aCGGCGA-GUCGGGUGcCgUCAUCGa -3' miRNA: 3'- uaGCCGCUgUAGCCCGCuG-AGUAGCc -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 10431 | 0.68 | 0.579505 |
Target: 5'- cUCGGCGAgcucgaCAUCGaGUGGCUCGaCGGc -3' miRNA: 3'- uAGCCGCU------GUAGCcCGCUGAGUaGCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 2068 | 0.68 | 0.579505 |
Target: 5'- -aCGGCGGuucaAUUGGGCGGgccugcucgauUUCAUCGGc -3' miRNA: 3'- uaGCCGCUg---UAGCCCGCU-----------GAGUAGCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 57444 | 0.68 | 0.590075 |
Target: 5'- -gCGGCGAgGUUGGuGCGACgcugCGGc -3' miRNA: 3'- uaGCCGCUgUAGCC-CGCUGaguaGCC- -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 60063 | 0.68 | 0.600679 |
Target: 5'- cUCGGgcucaGGC-UCGGGCGGCUCGcCGa -3' miRNA: 3'- uAGCCg----CUGuAGCCCGCUGAGUaGCc -5' |
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23459 | 5' | -56.6 | NC_005259.1 | + | 32141 | 0.68 | 0.632608 |
Target: 5'- -gCGGUGAUGUCGGGCuuauagaGCUUGUCGu -3' miRNA: 3'- uaGCCGCUGUAGCCCGc------UGAGUAGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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