Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23460 | 3' | -60.3 | NC_005259.1 | + | 68873 | 0.66 | 0.498722 |
Target: 5'- -cCGCGCCGGGC-GCgCCGAUg-AGGUa -3' miRNA: 3'- ccGCGCGGCCUGaUG-GGCUGagUCCA- -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 68711 | 0.68 | 0.40567 |
Target: 5'- aGCGCGUCGG--UGCCCGAgaaCUgGGGUc -3' miRNA: 3'- cCGCGCGGCCugAUGGGCU---GAgUCCA- -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 67425 | 0.66 | 0.508565 |
Target: 5'- cGGgGUGCCGGGCUcacGCuuGAgcgcgUCGGGg -3' miRNA: 3'- -CCgCGCGGCCUGA---UGggCUg----AGUCCa -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 65984 | 0.68 | 0.414484 |
Target: 5'- aGGCGCGCCGaGcaGCcGCUCGACgu-GGUg -3' miRNA: 3'- -CCGCGCGGC-C--UGaUGGGCUGaguCCA- -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 65892 | 0.76 | 0.12514 |
Target: 5'- cGGCGggacgguccCGCCGGACaagagauaaguUACCCGACUUGGGa -3' miRNA: 3'- -CCGC---------GCGGCCUG-----------AUGGGCUGAGUCCa -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 63686 | 0.74 | 0.175658 |
Target: 5'- cGGCGCGCCGGGgaucugcgcCUGCCCaGCggggaUCGGGg -3' miRNA: 3'- -CCGCGCGGCCU---------GAUGGGcUG-----AGUCCa -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 61506 | 0.66 | 0.548693 |
Target: 5'- aGGCGC-UCGGugUGCCgguCGACcucgUCGGGa -3' miRNA: 3'- -CCGCGcGGCCugAUGG---GCUG----AGUCCa -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 57694 | 0.75 | 0.15437 |
Target: 5'- uGGCcagguUGCCGGGCuUGCCCGACUCgcGGGUc -3' miRNA: 3'- -CCGc----GCGGCCUG-AUGGGCUGAG--UCCA- -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 56444 | 0.69 | 0.371621 |
Target: 5'- --aGCGCCGGGCcACgCaGCUCAGGg -3' miRNA: 3'- ccgCGCGGCCUGaUGgGcUGAGUCCa -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 55616 | 0.67 | 0.478337 |
Target: 5'- cGCGCGCCuuggccgggaucaGGGCcuguagacgaGCCCGuggGCUCAGGUc -3' miRNA: 3'- cCGCGCGG-------------CCUGa---------UGGGC---UGAGUCCA- -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 50410 | 0.76 | 0.118678 |
Target: 5'- aGCGCGCCGGugcCUugaCCGGCUCGGGc -3' miRNA: 3'- cCGCGCGGCCu--GAug-GGCUGAGUCCa -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 38298 | 0.67 | 0.441616 |
Target: 5'- cGGCGCugaucGUCGGACcgAUCUGugUCAuGGUg -3' miRNA: 3'- -CCGCG-----CGGCCUGa-UGGGCugAGU-CCA- -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 38027 | 0.66 | 0.508565 |
Target: 5'- aGGCccGCGuuGGugUACCCGGC-CAc-- -3' miRNA: 3'- -CCG--CGCggCCugAUGGGCUGaGUcca -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 35997 | 0.68 | 0.40567 |
Target: 5'- cGGCGagGCCGGACUGCaCCGA----GGUg -3' miRNA: 3'- -CCGCg-CGGCCUGAUG-GGCUgaguCCA- -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 34228 | 0.67 | 0.473544 |
Target: 5'- cGGCGCuGCCGGuCUugCCGuugaacguguccgacACaUCGGGc -3' miRNA: 3'- -CCGCG-CGGCCuGAugGGC---------------UG-AGUCCa -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 31510 | 0.7 | 0.316882 |
Target: 5'- -cCGCGCCGGACuUGCuuGA-UCGGGg -3' miRNA: 3'- ccGCGCGGCCUG-AUGggCUgAGUCCa -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 30829 | 0.67 | 0.441616 |
Target: 5'- cGGCGCGCCGaggucGGCaACCUGcacCUCAaGGUa -3' miRNA: 3'- -CCGCGCGGC-----CUGaUGGGCu--GAGU-CCA- -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 30796 | 0.71 | 0.288433 |
Target: 5'- cGCGUaccGCCGGAacACCUGAgUCGGGUa -3' miRNA: 3'- cCGCG---CGGCCUgaUGGGCUgAGUCCA- -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 28030 | 0.68 | 0.423415 |
Target: 5'- cGCGCGuuGGcCaGCCaCGGCUUGGGa -3' miRNA: 3'- cCGCGCggCCuGaUGG-GCUGAGUCCa -5' |
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23460 | 3' | -60.3 | NC_005259.1 | + | 27858 | 0.68 | 0.414484 |
Target: 5'- cGCGCGCCGuacGACcGCCCu-CUUGGGUg -3' miRNA: 3'- cCGCGCGGC---CUGaUGGGcuGAGUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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