Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23462 | 5' | -57.9 | NC_005259.1 | + | 49590 | 0.71 | 0.301309 |
Target: 5'- gGGCGGGGUCaGCAccucgaccucgggCAGCUCAUCAUc -3' miRNA: 3'- gUCGUCCCAGgCGUa------------GUCGAGUGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 28010 | 0.7 | 0.343073 |
Target: 5'- gGGCGGGGUCgccgggguugcgCGCGUUGGCcaGCCACg -3' miRNA: 3'- gUCGUCCCAG------------GCGUAGUCGagUGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 47887 | 0.69 | 0.421513 |
Target: 5'- uGGCcGGGUCaGCGUCGGCUCGuuGa -3' miRNA: 3'- gUCGuCCCAGgCGUAGUCGAGUggUg -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 13792 | 0.69 | 0.421513 |
Target: 5'- gAGCAGuGGUgCGaCAUCaccGGCgagCACCACg -3' miRNA: 3'- gUCGUC-CCAgGC-GUAG---UCGa--GUGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 61602 | 0.68 | 0.44996 |
Target: 5'- cCGGUGGGGUCgGCGgggaaCAGCagaccggCACCGCc -3' miRNA: 3'- -GUCGUCCCAGgCGUa----GUCGa------GUGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 29132 | 0.68 | 0.459675 |
Target: 5'- gCGGCGGuaUUCGCcUC-GCUCACCGCg -3' miRNA: 3'- -GUCGUCccAGGCGuAGuCGAGUGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 33117 | 0.68 | 0.479432 |
Target: 5'- -uGUAGGGcCaCGCggCAGC-CGCCGCg -3' miRNA: 3'- guCGUCCCaG-GCGuaGUCGaGUGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 14566 | 0.68 | 0.499595 |
Target: 5'- gAGCGGGGaCCGgcgaacacCAUCGcGCUgGCCACc -3' miRNA: 3'- gUCGUCCCaGGC--------GUAGU-CGAgUGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 24121 | 0.68 | 0.499595 |
Target: 5'- gGGCGGGGacuacaccgCCGCcgCccuGCUCGCCGa -3' miRNA: 3'- gUCGUCCCa--------GGCGuaGu--CGAGUGGUg -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 56373 | 0.68 | 0.499595 |
Target: 5'- aCAGCcgucgcGGG-CCGCGUCGGCUgAUCGu -3' miRNA: 3'- -GUCGu-----CCCaGGCGUAGUCGAgUGGUg -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 12133 | 0.67 | 0.520126 |
Target: 5'- aCAGCAccGGGUCUGCuuUCuugaUCACCGCc -3' miRNA: 3'- -GUCGU--CCCAGGCGu-AGucg-AGUGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 35852 | 0.67 | 0.530516 |
Target: 5'- gAGCAcaGGUCCGCcgCcGCgucCACCGCa -3' miRNA: 3'- gUCGUc-CCAGGCGuaGuCGa--GUGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 58691 | 0.67 | 0.530516 |
Target: 5'- -cGCGGGGUCgGCucGUC-GC-CGCCGCc -3' miRNA: 3'- guCGUCCCAGgCG--UAGuCGaGUGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 11027 | 0.67 | 0.551512 |
Target: 5'- aGGCAGGaGUUCGCcg-GGUggUCACCGCa -3' miRNA: 3'- gUCGUCC-CAGGCGuagUCG--AGUGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 46292 | 0.67 | 0.551512 |
Target: 5'- aCAGCGGGG-CCGCcgUGGCugcguuguUCGCgGCc -3' miRNA: 3'- -GUCGUCCCaGGCGuaGUCG--------AGUGgUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 49219 | 0.66 | 0.594184 |
Target: 5'- -uGCGGGGUuuacUCGuCGUCGGaCUCgACCGCu -3' miRNA: 3'- guCGUCCCA----GGC-GUAGUC-GAG-UGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 16717 | 0.66 | 0.594184 |
Target: 5'- gAGCAG-GUCgCGC-UCuGcCUCGCCACg -3' miRNA: 3'- gUCGUCcCAG-GCGuAGuC-GAGUGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 94 | 0.66 | 0.594184 |
Target: 5'- gAGgaaGGGGUcagacaugaCCG-AUCAGCUCACCAUc -3' miRNA: 3'- gUCg--UCCCA---------GGCgUAGUCGAGUGGUG- -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 27926 | 0.66 | 0.594184 |
Target: 5'- uGGUAGcGGUUCgGCGaccCAGCUCGCCGg -3' miRNA: 3'- gUCGUC-CCAGG-CGUa--GUCGAGUGGUg -5' |
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23462 | 5' | -57.9 | NC_005259.1 | + | 36639 | 0.66 | 0.604949 |
Target: 5'- -cGCAaGGcCCGCgAUCAGCgagcCGCCGCc -3' miRNA: 3'- guCGUcCCaGGCG-UAGUCGa---GUGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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