Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23463 | 3' | -53.5 | NC_005259.1 | + | 67054 | 0.7 | 0.639764 |
Target: 5'- aGCAcccgugCGCCGAUCUCaagGGCC--AGCg -3' miRNA: 3'- -CGUaa----GCGGCUAGAGca-CCGGcaUCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 61263 | 0.67 | 0.841114 |
Target: 5'- aGCAUgcgaccgUCGCUGGUCUCGauGCCGacgaacgcgAGCg -3' miRNA: 3'- -CGUA-------AGCGGCUAGAGCacCGGCa--------UCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 60500 | 0.66 | 0.849653 |
Target: 5'- aGCAcgCGCCGGUgUUGUcguagugcgccauGGCCG-GGCc -3' miRNA: 3'- -CGUaaGCGGCUAgAGCA-------------CCGGCaUCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 60469 | 0.67 | 0.833246 |
Target: 5'- ----aCGCUGGUCUCGUaGGCaCGcaccAGCg -3' miRNA: 3'- cguaaGCGGCUAGAGCA-CCG-GCa---UCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 60213 | 0.73 | 0.480597 |
Target: 5'- ---cUCGCCGAUCUCGUcGGCgcugAGCu -3' miRNA: 3'- cguaAGCGGCUAGAGCA-CCGgca-UCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 60079 | 0.67 | 0.824307 |
Target: 5'- gGCGgcUCGCCGAcuuUCUCGaUGGCg--AGCu -3' miRNA: 3'- -CGUa-AGCGGCU---AGAGC-ACCGgcaUCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 58433 | 0.7 | 0.65064 |
Target: 5'- gGCAgauugCGCUGAUCg-GUGGCCuUGGUg -3' miRNA: 3'- -CGUaa---GCGGCUAGagCACCGGcAUCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 56893 | 0.66 | 0.866848 |
Target: 5'- ----cCGCCaAUCgcagCGUGGCCGccGCa -3' miRNA: 3'- cguaaGCGGcUAGa---GCACCGGCauCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 50241 | 0.67 | 0.833246 |
Target: 5'- cGCGggCGCgGA--UCGUGGCCaacgGGCu -3' miRNA: 3'- -CGUaaGCGgCUagAGCACCGGca--UCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 49555 | 0.67 | 0.81517 |
Target: 5'- ----gUGCCGGgcugUUCGUcGGUCGUGGCc -3' miRNA: 3'- cguaaGCGGCUa---GAGCA-CCGGCAUCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 45514 | 0.69 | 0.725731 |
Target: 5'- gGCcaccgCGCCGGUgUUGgccGCCGUGGCu -3' miRNA: 3'- -CGuaa--GCGGCUAgAGCac-CGGCAUCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 37582 | 0.69 | 0.73514 |
Target: 5'- cGCccgCGCCGAUCugcaagcugaacgUCG-GGCCG-AGCu -3' miRNA: 3'- -CGuaaGCGGCUAG-------------AGCaCCGGCaUCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 31889 | 0.72 | 0.542656 |
Target: 5'- ---cUCGCCGGUCUCaaGGcCCGgcGCg -3' miRNA: 3'- cguaAGCGGCUAGAGcaCC-GGCauCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 31859 | 0.66 | 0.874671 |
Target: 5'- cGCGUcaUUGgCGAUCUUGUcGGUCGUGa- -3' miRNA: 3'- -CGUA--AGCgGCUAGAGCA-CCGGCAUcg -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 28960 | 0.71 | 0.585467 |
Target: 5'- cGCA-UCGCCGAggUCGgugccgaGGCCGcgAGCg -3' miRNA: 3'- -CGUaAGCGGCUagAGCa------CCGGCa-UCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 26435 | 0.66 | 0.866848 |
Target: 5'- cGCAc-CGCCGAg--CGggcgGGCCGgugGGCc -3' miRNA: 3'- -CGUaaGCGGCUagaGCa---CCGGCa--UCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 26125 | 0.67 | 0.824307 |
Target: 5'- cGCcggUCGCCGGUCcgacgccgcCGaGGCCG-AGCa -3' miRNA: 3'- -CGua-AGCGGCUAGa--------GCaCCGGCaUCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 24677 | 0.67 | 0.833246 |
Target: 5'- cCGUUCGCCaucCUCGUGcacguGCCG-AGCg -3' miRNA: 3'- cGUAAGCGGcuaGAGCAC-----CGGCaUCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 15421 | 0.67 | 0.824307 |
Target: 5'- uGCGUgccaccaagGCCGAUCUCGaguaucUGGCCGagaucaacccguUGGCc -3' miRNA: 3'- -CGUAag-------CGGCUAGAGC------ACCGGC------------AUCG- -5' |
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23463 | 3' | -53.5 | NC_005259.1 | + | 14729 | 0.66 | 0.858787 |
Target: 5'- cGCucgUCGCCGAgauguacgCGcUGGCCG-GGCc -3' miRNA: 3'- -CGua-AGCGGCUaga-----GC-ACCGGCaUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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