Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23463 | 5' | -61.6 | NC_005259.1 | + | 63724 | 0.68 | 0.359122 |
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Target: 5'- gGGCCUgcgcagcCGGUAgCGACauCUCGGCGGg- -3' miRNA: 3'- gCCGGA-------GCCGUgGCUG--GAGCCGCUac -5' |
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| 23463 | 5' | -61.6 | NC_005259.1 | + | 63939 | 0.67 | 0.418116 |
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Target: 5'- gGGUCUuaccgucCGGCAgCGAgaUCUCGGCGGUc -3' miRNA: 3'- gCCGGA-------GCCGUgGCU--GGAGCCGCUAc -5' |
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| 23463 | 5' | -61.6 | NC_005259.1 | + | 64774 | 0.68 | 0.387882 |
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Target: 5'- uGcGCCUCGGCggcccgcugcgacugGCugugugccuCGGCCUCGGCGGc- -3' miRNA: 3'- gC-CGGAGCCG---------------UG---------GCUGGAGCCGCUac -5' |
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| 23463 | 5' | -61.6 | NC_005259.1 | + | 67076 | 0.68 | 0.351971 |
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Target: 5'- gGGCCagCGuGaCGCCGACCUUGGCc--- -3' miRNA: 3'- gCCGGa-GC-C-GUGGCUGGAGCCGcuac -5' |
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| 23463 | 5' | -61.6 | NC_005259.1 | + | 67208 | 0.69 | 0.299844 |
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Target: 5'- -cGCCgcugUCGGCggggucgauGCCGACCaCGGCGGUGu -3' miRNA: 3'- gcCGG----AGCCG---------UGGCUGGaGCCGCUAC- -5' |
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| 23463 | 5' | -61.6 | NC_005259.1 | + | 67511 | 0.69 | 0.328871 |
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Target: 5'- --uUCUCGGCAgCGACCucUCGGCGGc- -3' miRNA: 3'- gccGGAGCCGUgGCUGG--AGCCGCUac -5' |
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| 23463 | 5' | -61.6 | NC_005259.1 | + | 67764 | 0.73 | 0.166206 |
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Target: 5'- cCGGCCacCGGcCACCGACCacgCGGUGAc- -3' miRNA: 3'- -GCCGGa-GCC-GUGGCUGGa--GCCGCUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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