Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23464 | 5' | -56.6 | NC_005259.1 | + | 15067 | 0.66 | 0.685185 |
Target: 5'- cCGUGgccACCCU-GGcCCUUGAGcCGGUc -3' miRNA: 3'- -GCGCau-UGGGAgCC-GGAACUCuGCCA- -5' |
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23464 | 5' | -56.6 | NC_005259.1 | + | 62518 | 0.66 | 0.706241 |
Target: 5'- aGCGgucagcacuUCCUCGGCCUc--GACGGUc -3' miRNA: 3'- gCGCauu------GGGAGCCGGAacuCUGCCA- -5' |
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23464 | 5' | -56.6 | NC_005259.1 | + | 27023 | 0.66 | 0.715633 |
Target: 5'- gCGcCGUAGCUgaacaccuugagCUUGGCCUUGGccugagcGACGGUc -3' miRNA: 3'- -GC-GCAUUGG------------GAGCCGGAACU-------CUGCCA- -5' |
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23464 | 5' | -56.6 | NC_005259.1 | + | 47307 | 0.66 | 0.716673 |
Target: 5'- aGCGac-CCCUUGGCCU-GAccGGCGGc -3' miRNA: 3'- gCGCauuGGGAGCCGGAaCU--CUGCCa -5' |
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23464 | 5' | -56.6 | NC_005259.1 | + | 24270 | 0.67 | 0.641504 |
Target: 5'- aCGCGUGGCUggaagcgCUCGaCCUcGGGAUGGUg -3' miRNA: 3'- -GCGCAUUGG-------GAGCcGGAaCUCUGCCA- -5' |
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23464 | 5' | -56.6 | NC_005259.1 | + | 59406 | 0.67 | 0.675641 |
Target: 5'- aGCGUGACCUcgcgcauggcgucggCGGCCaUGAagaacGGCGGUu -3' miRNA: 3'- gCGCAUUGGGa--------------GCCGGaACU-----CUGCCA- -5' |
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23464 | 5' | -56.6 | NC_005259.1 | + | 42634 | 0.7 | 0.466061 |
Target: 5'- gCGCGUAgGCaCCUCGGCCccgaUGuGGGCGGc -3' miRNA: 3'- -GCGCAU-UG-GGAGCCGGa---AC-UCUGCCa -5' |
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23464 | 5' | -56.6 | NC_005259.1 | + | 64799 | 0.7 | 0.475858 |
Target: 5'- gGCuGUGugCCUCGGCCUc--GGCGGc -3' miRNA: 3'- gCG-CAUugGGAGCCGGAacuCUGCCa -5' |
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23464 | 5' | -56.6 | NC_005259.1 | + | 59054 | 0.71 | 0.400634 |
Target: 5'- gCGCGUccucaggggucaGGCUCUcacCGGCCUUGuGGCGGg -3' miRNA: 3'- -GCGCA------------UUGGGA---GCCGGAACuCUGCCa -5' |
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23464 | 5' | -56.6 | NC_005259.1 | + | 29932 | 0.73 | 0.333433 |
Target: 5'- aCGCGUGgccgccacgcgGCCCggucgCGGCUggUGGGGCGGg -3' miRNA: 3'- -GCGCAU-----------UGGGa----GCCGGa-ACUCUGCCa -5' |
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23464 | 5' | -56.6 | NC_005259.1 | + | 8895 | 0.74 | 0.295815 |
Target: 5'- aGCGUcACgCUCGGCCUgcuGACGGg -3' miRNA: 3'- gCGCAuUGgGAGCCGGAacuCUGCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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