Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23465 | 5' | -54.7 | NC_005259.1 | + | 15159 | 0.78 | 0.158178 |
Target: 5'- gUGGCCgaGGCCgaGGCCAUCcUCGAc -3' miRNA: 3'- -ACCGGaaCCGGaaCCGGUAGuAGUUa -5' |
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23465 | 5' | -54.7 | NC_005259.1 | + | 45497 | 0.73 | 0.348505 |
Target: 5'- gUGGCCUUGGUCgcugUGGCCAccgCGcCGGUg -3' miRNA: 3'- -ACCGGAACCGGa---ACCGGUa--GUaGUUA- -5' |
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23465 | 5' | -54.7 | NC_005259.1 | + | 58955 | 0.71 | 0.440467 |
Target: 5'- cGGCCUUGGCCagcaGGUCGggcagcUCGUCGGc -3' miRNA: 3'- aCCGGAACCGGaa--CCGGU------AGUAGUUa -5' |
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23465 | 5' | -54.7 | NC_005259.1 | + | 27047 | 0.7 | 0.501819 |
Target: 5'- uUGGCCUUGGCCUgagcgacGGUCAcggcgaCAUCGc- -3' miRNA: 3'- -ACCGGAACCGGAa------CCGGUa-----GUAGUua -5' |
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23465 | 5' | -54.7 | NC_005259.1 | + | 60131 | 0.69 | 0.555647 |
Target: 5'- cGGCCUcguccugcgcGGCCUUGGCCGcCGccUCAGc -3' miRNA: 3'- aCCGGAa---------CCGGAACCGGUaGU--AGUUa -5' |
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23465 | 5' | -54.7 | NC_005259.1 | + | 44277 | 0.68 | 0.644551 |
Target: 5'- aGGCCgaacGCCUUGGCCAgCG-CGAg -3' miRNA: 3'- aCCGGaac-CGGAACCGGUaGUaGUUa -5' |
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23465 | 5' | -54.7 | NC_005259.1 | + | 47316 | 0.68 | 0.644551 |
Target: 5'- uUGGCCUgaccggcGGCCUUGGUCAgccccgugaUCGUUu-- -3' miRNA: 3'- -ACCGGAa------CCGGAACCGGU---------AGUAGuua -5' |
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23465 | 5' | -54.7 | NC_005259.1 | + | 65793 | 0.68 | 0.644551 |
Target: 5'- aUGGCCUcgacgcgcUGGUCgaaacgcUGGCCGUCGaccUCGAa -3' miRNA: 3'- -ACCGGA--------ACCGGa------ACCGGUAGU---AGUUa -5' |
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23465 | 5' | -54.7 | NC_005259.1 | + | 45161 | 0.67 | 0.655716 |
Target: 5'- aGGCCgcccUUGGCCUUGuugcgcGCCGagGUCAGc -3' miRNA: 3'- aCCGG----AACCGGAAC------CGGUagUAGUUa -5' |
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23465 | 5' | -54.7 | NC_005259.1 | + | 8758 | 0.66 | 0.710982 |
Target: 5'- aGGUCgaGGcCCUUGGCCAgcgCggCGGUg -3' miRNA: 3'- aCCGGaaCC-GGAACCGGUa--GuaGUUA- -5' |
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23465 | 5' | -54.7 | NC_005259.1 | + | 4459 | 0.66 | 0.753823 |
Target: 5'- gGGCagcguaugGGCCaaGGCCAUCggCGGUg -3' miRNA: 3'- aCCGgaa-----CCGGaaCCGGUAGuaGUUA- -5' |
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23465 | 5' | -54.7 | NC_005259.1 | + | 47871 | 0.66 | 0.753823 |
Target: 5'- uUGGCCUcuuccagcuUGGCCg-GGUCAgCGUCGGc -3' miRNA: 3'- -ACCGGA---------ACCGGaaCCGGUaGUAGUUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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