Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23467 | 3' | -56.3 | NC_005259.1 | + | 56084 | 0.66 | 0.70358 |
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Target: 5'- aGCAGAUCGGUgCUCGucggGGCCaccgcGUGGCc- -3' miRNA: 3'- gCGUCUAGCUA-GGGU----UCGG-----CACCGac -5' |
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| 23467 | 3' | -56.3 | NC_005259.1 | + | 10476 | 0.68 | 0.574697 |
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Target: 5'- aGCGGGaCGAUCUUggGgUCGUGGUUGu -3' miRNA: 3'- gCGUCUaGCUAGGGuuC-GGCACCGAC- -5' |
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| 23467 | 3' | -56.3 | NC_005259.1 | + | 66254 | 0.68 | 0.564047 |
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Target: 5'- aCGCAGcgCGG-CCCGcGCCG-GGCg- -3' miRNA: 3'- -GCGUCuaGCUaGGGUuCGGCaCCGac -5' |
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| 23467 | 3' | -56.3 | NC_005259.1 | + | 33306 | 0.71 | 0.423306 |
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Target: 5'- uGCGGcUCGAUCCC--GCCGUcGGCg- -3' miRNA: 3'- gCGUCuAGCUAGGGuuCGGCA-CCGac -5' |
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| 23467 | 3' | -56.3 | NC_005259.1 | + | 5499 | 0.72 | 0.387925 |
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Target: 5'- aGCgaGGAUCGAgcuacgcgcgccgcgCCCGcaAGCUGUGGCUGc -3' miRNA: 3'- gCG--UCUAGCUa--------------GGGU--UCGGCACCGAC- -5' |
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| 23467 | 3' | -56.3 | NC_005259.1 | + | 30432 | 0.74 | 0.284078 |
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Target: 5'- gGCAGcgCGAU---GAGCCGUGGCUGu -3' miRNA: 3'- gCGUCuaGCUAgggUUCGGCACCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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