Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23468 | 5' | -55.3 | NC_005259.1 | + | 2157 | 0.72 | 0.380721 |
Target: 5'- uGGcCAGCGGCGUcGAgccgccgguUGCCGAGGUCa- -3' miRNA: 3'- gUC-GUCGCCGCA-UU---------ACGGCUCUAGgu -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 7032 | 0.66 | 0.773385 |
Target: 5'- gAGCgccccGGCGGgGUuuuucguUGCgCGAGAUCCu -3' miRNA: 3'- gUCG-----UCGCCgCAuu-----ACG-GCUCUAGGu -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 7239 | 0.68 | 0.602349 |
Target: 5'- aCGGCAGCcGCGccGGUGUCGAGugcgCCAa -3' miRNA: 3'- -GUCGUCGcCGCa-UUACGGCUCua--GGU- -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 8220 | 0.66 | 0.729155 |
Target: 5'- aCAGCgAGCGuGCgGUGGUGCCGAcacccugccuuaccGGUCUu -3' miRNA: 3'- -GUCG-UCGC-CG-CAUUACGGCU--------------CUAGGu -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 14524 | 0.71 | 0.47548 |
Target: 5'- gAGCAcGCGGUGcguuucGGUGCCGAGuucGUCCGg -3' miRNA: 3'- gUCGU-CGCCGCa-----UUACGGCUC---UAGGU- -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 16155 | 0.67 | 0.689643 |
Target: 5'- aGGUuGUGGUGUccacucccgugGccGCCGAGGUCCAa -3' miRNA: 3'- gUCGuCGCCGCA-----------UuaCGGCUCUAGGU- -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 24725 | 0.67 | 0.70042 |
Target: 5'- uCGGCGaucacgauugauGCGGUGUGggGUGCCGAGGcgcucaucgaaUCCu -3' miRNA: 3'- -GUCGU------------CGCCGCAU--UACGGCUCU-----------AGGu -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 26818 | 0.67 | 0.667938 |
Target: 5'- cCGGUAcCGGCgGUGGUGCCGGGGcggUCUg -3' miRNA: 3'- -GUCGUcGCCG-CAUUACGGCUCU---AGGu -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 27402 | 0.66 | 0.721763 |
Target: 5'- uCGGCAGCGGCGgcagcucGGUGCCcgcGAGGa--- -3' miRNA: 3'- -GUCGUCGCCGCa------UUACGG---CUCUaggu -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 28237 | 0.7 | 0.485604 |
Target: 5'- uCGGCGGCGGgGUG--GUCGAucacGAUCCAu -3' miRNA: 3'- -GUCGUCGCCgCAUuaCGGCU----CUAGGU- -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 39764 | 0.67 | 0.689643 |
Target: 5'- gCAGCAGCGGCagccugcugcugGUGAUcggugcGCCGAGcucaagCCGa -3' miRNA: 3'- -GUCGUCGCCG------------CAUUA------CGGCUCua----GGU- -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 44956 | 0.7 | 0.516562 |
Target: 5'- gGGCAguGCGGUcaucggGAUGCCGAGggCCGg -3' miRNA: 3'- gUCGU--CGCCGca----UUACGGCUCuaGGU- -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 50435 | 0.69 | 0.580588 |
Target: 5'- gGGCAGCGGUGcgg-GCuCGGGGUCg- -3' miRNA: 3'- gUCGUCGCCGCauuaCG-GCUCUAGgu -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 55339 | 0.73 | 0.338307 |
Target: 5'- gCGGCAGCGGUGgcGAUGCCGAacgCCu -3' miRNA: 3'- -GUCGUCGCCGCa-UUACGGCUcuaGGu -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 61223 | 0.66 | 0.732309 |
Target: 5'- aAGCGGCGGUGUGcgcaCCGAGAaCUc -3' miRNA: 3'- gUCGUCGCCGCAUuac-GGCUCUaGGu -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 61926 | 0.66 | 0.773385 |
Target: 5'- uCGGCAGCucggucucucGCGcgGGUGCCGGGG-CCAc -3' miRNA: 3'- -GUCGUCGc---------CGCa-UUACGGCUCUaGGU- -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 63305 | 0.68 | 0.613272 |
Target: 5'- gAGCAGCGGgGUcagGAUGUCGuuguAGAUCg- -3' miRNA: 3'- gUCGUCGCCgCA---UUACGGC----UCUAGgu -5' |
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23468 | 5' | -55.3 | NC_005259.1 | + | 67977 | 0.67 | 0.71113 |
Target: 5'- -cGCAGCGGCGUGGccaccGCCGcGAgcgUCGa -3' miRNA: 3'- guCGUCGCCGCAUUa----CGGCuCUa--GGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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