Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23469 | 3' | -55.2 | NC_005259.1 | + | 10253 | 0.66 | 0.733216 |
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Target: 5'- gGUGUGCUUGAUgagcGGGCCGgucUCAGGc-- -3' miRNA: 3'- -CACACGAGCUGa---CCCGGU---GGUUCuuu -5' |
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| 23469 | 3' | -55.2 | NC_005259.1 | + | 27883 | 0.67 | 0.634885 |
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Target: 5'- gGUGUGCaccaCGAUccaccugGGGUCGCCGAGAc- -3' miRNA: 3'- -CACACGa---GCUGa------CCCGGUGGUUCUuu -5' |
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| 23469 | 3' | -55.2 | NC_005259.1 | + | 66929 | 0.68 | 0.590643 |
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Target: 5'- -gGUGCUCGACcgGGGUgAgCGGGAc- -3' miRNA: 3'- caCACGAGCUGa-CCCGgUgGUUCUuu -5' |
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| 23469 | 3' | -55.2 | NC_005259.1 | + | 63550 | 0.69 | 0.53619 |
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Target: 5'- -gGUGCUCGGC--GGCCACCGAc--- -3' miRNA: 3'- caCACGAGCUGacCCGGUGGUUcuuu -5' |
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| 23469 | 3' | -55.2 | NC_005259.1 | + | 42769 | 0.69 | 0.53619 |
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Target: 5'- --cUGCUCGGCggucucgGGGCCGCCAc---- -3' miRNA: 3'- cacACGAGCUGa------CCCGGUGGUucuuu -5' |
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| 23469 | 3' | -55.2 | NC_005259.1 | + | 30152 | 0.7 | 0.473363 |
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Target: 5'- ---aGCUCGACcGGGCCGCCuacAGGc- -3' miRNA: 3'- cacaCGAGCUGaCCCGGUGGu--UCUuu -5' |
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| 23469 | 3' | -55.2 | NC_005259.1 | + | 56092 | 0.71 | 0.405082 |
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Target: 5'- -gGUGCUCGuCgGGGCCACCGc---- -3' miRNA: 3'- caCACGAGCuGaCCCGGUGGUucuuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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