miRNA: 3'- uaGGACG-----GGCUCGUCuGGCGGGgCCG- -5'
miRNA: 3'- uaGGa-CGG-GCuC-GUCugGCGGGGCCG- -5'
miRNA: 3'- -UAGGacg-GGC--UCG----UCUGGCGGGGCCG- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23469 | 5' | -64.2 | NC_005259.1 | + | 63418 | 0.71 | 0.153426 |
|
Target: 5'- -cCUUGCucaugCCGAGCAG-CCGCUCgGGCg -3' miRNA: 3'- uaGGACG-----GGCUCGUCuGGCGGGgCCG- -5' |
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| 23469 | 5' | -64.2 | NC_005259.1 | + | 64757 | 0.66 | 0.342567 |
|
Target: 5'- -aCCgcGCCuCGcGaCGGugCGCCUCGGCg -3' miRNA: 3'- uaGGa-CGG-GCuC-GUCugGCGGGGCCG- -5' |
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| 23469 | 5' | -64.2 | NC_005259.1 | + | 67735 | 0.72 | 0.121442 |
|
Target: 5'- gAUCCgauaCCGgcGGCcacuAGGCCGCCCCGGCc -3' miRNA: 3'- -UAGGacg-GGC--UCG----UCUGGCGGGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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