Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2347 | 3' | -55.6 | NC_001416.1 | + | 2864 | 0.66 | 0.666907 |
Target: 5'- gGGCCGGACgGC-AUGacaucGCUGCGCg-- -3' miRNA: 3'- gUCGGCCUGgCGaUAU-----UGGCGUGagc -5' |
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2347 | 3' | -55.6 | NC_001416.1 | + | 5438 | 0.66 | 0.666907 |
Target: 5'- gCAGCCGGACgugaaCGCgcagAUcACCGCAg-CGg -3' miRNA: 3'- -GUCGGCCUG-----GCGa---UAuUGGCGUgaGC- -5' |
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2347 | 3' | -55.6 | NC_001416.1 | + | 17554 | 0.67 | 0.600531 |
Target: 5'- -cGuCCGGGCCGUgcUGACCaGcCGCUCa -3' miRNA: 3'- guC-GGCCUGGCGauAUUGG-C-GUGAGc -5' |
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2347 | 3' | -55.6 | NC_001416.1 | + | 19057 | 0.68 | 0.52442 |
Target: 5'- gGGCCGGAgUGaCUGUcACCGCAagugCGg -3' miRNA: 3'- gUCGGCCUgGC-GAUAuUGGCGUga--GC- -5' |
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2347 | 3' | -55.6 | NC_001416.1 | + | 16423 | 0.69 | 0.452267 |
Target: 5'- -uGCCGGACgGCUuUGGCgGCACg-- -3' miRNA: 3'- guCGGCCUGgCGAuAUUGgCGUGagc -5' |
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2347 | 3' | -55.6 | NC_001416.1 | + | 1915 | 0.71 | 0.359462 |
Target: 5'- cCAGCUGGACCGCUAcgAAauGCGCg-- -3' miRNA: 3'- -GUCGGCCUGGCGAUa-UUggCGUGagc -5' |
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2347 | 3' | -55.6 | NC_001416.1 | + | 5283 | 0.72 | 0.334336 |
Target: 5'- uGGCCuccuGACCGCUGUAcACUGCAgcCUCGg -3' miRNA: 3'- gUCGGc---CUGGCGAUAU-UGGCGU--GAGC- -5' |
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2347 | 3' | -55.6 | NC_001416.1 | + | 43972 | 0.75 | 0.228221 |
Target: 5'- aAGCCGGAuUCGgUAUGGCUGCAUUCu -3' miRNA: 3'- gUCGGCCU-GGCgAUAUUGGCGUGAGc -5' |
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2347 | 3' | -55.6 | NC_001416.1 | + | 9483 | 0.75 | 0.199762 |
Target: 5'- -uGCCGGuuGCCGCUGUuACCGUGCUgCGa -3' miRNA: 3'- guCGGCC--UGGCGAUAuUGGCGUGA-GC- -5' |
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2347 | 3' | -55.6 | NC_001416.1 | + | 4688 | 0.84 | 0.048242 |
Target: 5'- uCAGCCGGACgcgggCGCUGcAGCCGUACUCGg -3' miRNA: 3'- -GUCGGCCUG-----GCGAUaUUGGCGUGAGC- -5' |
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2347 | 3' | -55.6 | NC_001416.1 | + | 14028 | 1.08 | 0.000874 |
Target: 5'- aCAGCCGGACCGCUAUAACCGCACUCGu -3' miRNA: 3'- -GUCGGCCUGGCGAUAUUGGCGUGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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