Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23470 | 3' | -57.7 | NC_005259.1 | + | 59319 | 0.66 | 0.631272 |
Target: 5'- gCGGCGaGCUcagccucGCguuucgcuaccgcauAGUCgUCGGUGGGAUCg -3' miRNA: 3'- aGCCGC-CGA-------UG---------------UCGG-AGCCACUCUAG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 1236 | 0.66 | 0.628058 |
Target: 5'- -aGGaCGGCUGCaagaccGGCCUCGGcaucaccucGGAUCa -3' miRNA: 3'- agCC-GCCGAUG------UCGGAGCCac-------UCUAG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 64213 | 0.67 | 0.595968 |
Target: 5'- cUCGcCGGUcgGCAGCC-CGGUaucGGGGUCg -3' miRNA: 3'- -AGCcGCCGa-UGUCGGaGCCA---CUCUAG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 58797 | 0.67 | 0.595968 |
Target: 5'- -aGGCGGUgauuGCCUCGGcGGGcUCg -3' miRNA: 3'- agCCGCCGauguCGGAGCCaCUCuAG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 50514 | 0.67 | 0.594902 |
Target: 5'- gCGGUGcGCUGCAccucgacgcgcucGCCUCGGgcGAGGa- -3' miRNA: 3'- aGCCGC-CGAUGU-------------CGGAGCCa-CUCUag -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 46441 | 0.67 | 0.585319 |
Target: 5'- aUGGUGGCaaGCuGCugCUCGGUGAGGcUCa -3' miRNA: 3'- aGCCGCCGa-UGuCG--GAGCCACUCU-AG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 19963 | 0.67 | 0.585319 |
Target: 5'- aCGGCGGCUcgGCGGUgUgGGUGcccGUCu -3' miRNA: 3'- aGCCGCCGA--UGUCGgAgCCACuc-UAG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 40582 | 0.67 | 0.585319 |
Target: 5'- gUCGGCGGCUcgcacCAGCacaUCGuUGuAGGUCu -3' miRNA: 3'- -AGCCGCCGAu----GUCGg--AGCcAC-UCUAG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 52382 | 0.67 | 0.574706 |
Target: 5'- --aGCGGCUGCGcagccuGCCUCGGgccaucGAGGUg -3' miRNA: 3'- agcCGCCGAUGU------CGGAGCCa-----CUCUAg -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 58942 | 0.67 | 0.574706 |
Target: 5'- gCGGUGGCagcguCGGCCUUGGccaGcAGGUCg -3' miRNA: 3'- aGCCGCCGau---GUCGGAGCCa--C-UCUAG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 67900 | 0.67 | 0.564139 |
Target: 5'- uUCGGCuGUg--GGUCUCGGcGAGGUCa -3' miRNA: 3'- -AGCCGcCGaugUCGGAGCCaCUCUAG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 41637 | 0.67 | 0.542125 |
Target: 5'- gUGGCGGUgccggucUGCgGGCgCUCGGUGAgcggGAUCu -3' miRNA: 3'- aGCCGCCG-------AUG-UCG-GAGCCACU----CUAG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 27093 | 0.68 | 0.522457 |
Target: 5'- gCGGCGGCU---GCCaugCGGUGGGcuguGUCa -3' miRNA: 3'- aGCCGCCGAuguCGGa--GCCACUC----UAG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 19840 | 0.68 | 0.522457 |
Target: 5'- gCGGCuGCUGCcGCgCUCGGUGuGGcgcUCg -3' miRNA: 3'- aGCCGcCGAUGuCG-GAGCCACuCU---AG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 23618 | 0.68 | 0.491992 |
Target: 5'- gUGGCGGCagccagACGG-CUCGGUGuGGUUu -3' miRNA: 3'- aGCCGCCGa-----UGUCgGAGCCACuCUAG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 17361 | 0.68 | 0.482019 |
Target: 5'- gUCGGCGGCU-CGGCgCU-GGUcGuGGUCg -3' miRNA: 3'- -AGCCGCCGAuGUCG-GAgCCA-CuCUAG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 13117 | 0.7 | 0.424398 |
Target: 5'- cUCGGCGGCUACgaccAGCgCaCGGUGuucggcucGAUCa -3' miRNA: 3'- -AGCCGCCGAUG----UCG-GaGCCACu-------CUAG- -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 783 | 0.7 | 0.406122 |
Target: 5'- -aGGCGGCUGC--CCUCGGUuGGGUg -3' miRNA: 3'- agCCGCCGAUGucGGAGCCAcUCUAg -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 8369 | 0.7 | 0.379655 |
Target: 5'- cUCGGCGGCggccuCGGCaUCGGcGAGAg- -3' miRNA: 3'- -AGCCGCCGau---GUCGgAGCCaCUCUag -5' |
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23470 | 3' | -57.7 | NC_005259.1 | + | 49677 | 0.71 | 0.354369 |
Target: 5'- cUCGGCcuGGUaguCGGCCucgcgcucgcugUCGGUGAGAUCc -3' miRNA: 3'- -AGCCG--CCGau-GUCGG------------AGCCACUCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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